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Chitosan Film Containing Poly(D,L-Lactic-Co-Glycolic Acid) Nanoparticles: A Platform for Localized Dual-Drug Release

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ABSTRACT

Purpose

To characterize and evaluate chitosan film containing PLGA nanoparticles (NPs) as a platform for localized dual-drug release.

Methods

Fluorescent Paclitaxel (FPTX), a hydrophobic drug, was incorporated into PLGA NPs. FPTX-loaded PLGA NPs and Carboxyfluorescein (CF), a hydrophilic model drug, were embedded into chitosan films. Release of CF and NPs from chitosan and release of FPTX from PLGA NPs were monitored by fluorescence. The stability of the platform was observed through SEM and dynamic light scattering (DLS).

Results

Chitosan films containing CF and FPTX-loaded PLGA NPs showed a biphasic release profile. In the first phase, 78% of CF and 34% of NPs were released within few days. In the second phase, the release was slower, showing an additional release of 22% of CF and 18% of NPs after 3 weeks. SEM images and DLS measurements showed that NP release depends on film degradation rate. FPTX-loaded PLGA NPs showed the release of 19.8% of total drug in 2 days, and no additional release was detected in the next 26 days.

Conclusions

The ability of chitosan film containing PLGA NPs to coat gold surface and to incorporate and release two different drugs of different hydrophilicity make it a promising platform for localized dual-drug release.

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ACKNOWLEDGEMENTS

The authors thank Dr. Bernard D. F. Casse, William Fowle and Electronic Materials Research Institute of Northeastern University for SEM images. This work was supported by the IGERT Nanomedicine Science and Technology Program (NSF-0504331) and Brigham Womens Hospital.

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Authors and Affiliations

  1. Electronic Materials Research Institute and Department of Physics, Northeastern University, Boston, Massachusetts, USA

    Dayane B. Tada, Dattatri Nagesha, Evan Jost, Craig O. Levy, Evin Gultepe & Srinivas Sridhar

  2. Department of Engineering Science and Materials, University of Puerto Rico, Mayaguez, PR-00681, Puerto Rico

    Surinder Singh

  3. Department of Radiation Oncology, Dana Farber Cancer Institute Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA

    Robert Cormack & G. Mike Makrigiorgos

Authors
  1. Dayane B. Tada
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  2. Surinder Singh
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  3. Dattatri Nagesha
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  4. Evan Jost
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  5. Craig O. Levy
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  6. Evin Gultepe
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  9. Srinivas Sridhar
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Correspondence to Srinivas Sridhar.

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Tada, D.B., Singh, S., Nagesha, D. et al. Chitosan Film Containing Poly(D,L-Lactic-Co-Glycolic Acid) Nanoparticles: A Platform for Localized Dual-Drug Release. Pharm Res 27, 1738–1745 (2010). https://doi.org/10.1007/s11095-010-0176-9

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  • DOI: https://doi.org/10.1007/s11095-010-0176-9

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