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Active Blood Vessel Formation in the Ischemic Hindlimb Mouse Model Using a Microsphere/Hydrogel Combination System

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Abstract

Purpose

We hypothesize that the controlled delivery of rhVEGF using a microsphere/hydrogel combination system could be useful to achieve active blood vessel formation in the ischemic hindlimb mouse model, which is clinically relevant for therapeutic angiogenesis without multiple administrations.

Methods

A combination of poly(d,l-lactide-co-glycolide) (PLGA) microspheres and alginate hydrogels containing rhVEGF was prepared and injected intramuscularly into the ischemic hindlimb site of mouse model, and new blood vessel formation near the ischemic site was evaluated.

Results

The controlled release of rhVEGF from the combination system effectively protected muscles in ischemic regions from tissue necrosis. Interestingly, the number of newly formed, active blood vessels was significantly increased in mice treated with the rhVEGF-releasing combination system.

Conclusion

A microsphere/hydrogel combination system provided a useful means to deliver therapeutic angiogenic molecules into the body for the treatment of ischemic vascular diseases, which could reduce the number of administrations of many types of drugs.

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Acknowledgments

This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (2009-K001598, 2009-0065528), and also by grant from World Class University Project, Ministry of Education, Science and Technology, Republic of Korea (200900000000024). Authors acknowledge Byung-Gee Kim and Eunjung Song for their assistance in western blot analysis.

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Authors and Affiliations

  1. Department of Bioengineering, Hanyang University, Seoul, 133-791, Republic of Korea

    Jangwook Lee, Honghyun Park & Kuen Yong Lee

  2. School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744, Republic of Korea

    Suk Ho Bhang & Byung-Soo Kim

Authors
  1. Jangwook Lee
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  2. Suk Ho Bhang
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  3. Honghyun Park
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  4. Byung-Soo Kim
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  5. Kuen Yong Lee
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Corresponding authors

Correspondence to Byung-Soo Kim or Kuen Yong Lee.

Additional information

Authors Jangwook Lee and Suk Ho Bhang contributed equally to this work.

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Lee, J., Bhang, S.H., Park, H. et al. Active Blood Vessel Formation in the Ischemic Hindlimb Mouse Model Using a Microsphere/Hydrogel Combination System. Pharm Res 27, 767–774 (2010). https://doi.org/10.1007/s11095-010-0067-0

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  • DOI: https://doi.org/10.1007/s11095-010-0067-0

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