Investigation of Polylactic Acid (PLA) Nanoparticles as Drug Delivery Systems for Local Dermatotherapy
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The development of particle-based carriers for transepidermal drug delivery has become a field of major interest in dermatology. In this study, we investigated the suitability of biodegradable poly-lactic acid (PLA) particles loaded with fluorescent dyes as carriers for transepidermal drug delivery.
The penetration profiles of PLA particles (228 and 365 nm) and the release of dye from the particles were investigated in human skin explants using fluorescence microscopy, confocal laser scanning microscopy and flow cytometry.
PLA particles penetrated into 50% of the vellus hair follicles, reaching a maximal depth corresponding to the entry of the sebaceous gland in 12–15% of all observed follicles. The accumulation of particles in the follicular ducts was accompanied by the release of dye to the viable epidermis and its retention in the sebaceous glands for up to 24 h. Kinetic studies in vitro as well as in skin explants revealed, that, although stable in aqueous solution, destabilization of the particles and significant release of incorporated dye occurred upon contact with organic solvents and the skin surface.
These results suggest that particles based on PLA polymers may be ideal carriers for hair follicle and sebaceous gland targeting.
Key wordsdrug delivery nanoparticles poly-lactic acid skin penetration sebaceous gland
coumarin-6-loaded 365 nm PLA particles
cyanoacrylate skin surface stripping
mean fluorescence intensity
nile red-loaded 228 nm PLA particles
solid lipid nanoparticles
vellus hair follicle
This work was partially supported by European funding to B. Verrier through the FP6 STREP Munanovac programme. C. Primard was supported by a fellowship from the Region Rhone-Alpes (France).