Skip to main content
SpringerLink
Log in

HPTLC Method Development for Estimation of Vasicine in Herbal Extract and its Marketed Formulations

  • Published:
Pharmaceutical Chemistry Journal Aims and scope

Adhatoda vasica Nees, is locally known as adulsa, which is used in various medicinal activities. Hence, an objective of the study was to develop an high-performance thin-layer chromatography method for estimation of vasicine in herbal extract and its marketed herbal formulation. The current study focuses on the stability study for estimation of vasicine by a newly developed, less time-consuming method. In the present study, dried vasaka leaf powder was extracted with 90% methanol to obtain methanolic extract. The method was optimized for the dried methanolic extract using silica gel 60 F254 precoated thin-layer chromatography aluminum plates as the stationary phase and methanol: toluene (8.5:1.5 v/v) as the mobile phase. Detection was carried out in the absorbance mode at 286 nm. The developed method was validated and the extract was subjected to forced degradation studies using acid, alkali, and oxidative, thermolytic, and photolytic degradation as per International Council on Harmonization guidelines. The optimized method gave an Rf value of 0.27 ± 0.02. A good linear relationship was achieved for vasicine in the linear regression data for the calibration curves over the concentration range of 600 – 3600 ng/band (R2 = 0.9992). The limit of detection and limit of quantitation were found to be 200 ng/spot and 400 ng/spot respectively. The percentage content of vasicine was obtained (99.91% for the marketed syrup formulation and 99.87% for the marketed tablet formulation. The developed method gave a good result, which shows its applicability in the determination of vasicine in extract and its various marketed formulations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

References

  1. S. K. Singh, J. R. Patel, A. Dangi, et al. , J. Med. Plants Studies, 5, 175 – 180 (2017).

    Google Scholar 

  2. N. Kumar, World J. Pharm. Res., 5, 437 – 455 (2016).

    CAS  Google Scholar 

  3. V. Gayathri, V. Bindhu, Ind. J. Pharm. Pharm. Res., 6, 541 – 547 (2016).

    CAS  Google Scholar 

  4. V. V. Kadlag, V. S. Kasture, S. A. Gosavi, et al., Asian J. Chem., 23, 1917 – 1921 (2011).

    CAS  Google Scholar 

  5. B. K. Sajeeb, U. Kumar, A. S. S. Rouf, et al., Ind. J. Trad. Knowledge, 14, 637 – 642 (2015).

    Google Scholar 

  6. S. Soni, S. Anandjiwala, G. Patel, et al., Ind. J. Pharm. Sci., 70, 36 – 42 (2008); DOI: https://doi.org/10.4103/0250-474X.40329.

  7. P. Ravi Kumar, World J. Pharm. Pharmaceut. Sci., 4, 1075 – 1083 (2015).

  8. S. K. Dinakaran, S. Chelle, H. AvasaralaHYPERLINK “https://www.sciencedirect.com/science/article/pii/S2225411017301335” \l “!” , J. Trad. Complementary Med., 9, 319 – 327 (2019). https://doi.org/10.1016/j.jtcme.2017.12.001.

  9. B. K. Sajeeb, U. Kumar, Md. S. Hossain, et al., Dhaka Univ. J. Parm. Sci., 15, 57 – 62 (2016).

    Article  CAS  Google Scholar 

  10. A. Sharma, K. Lal, S. Handa, Pharma Bio, 30, 205 – 208 (2008). https://doi.org/10.3109/13880209209053998

    Article  Google Scholar 

  11. H. N. A. Ram, A. Shirwaikar, Pharmacology online, 2, 53 – 60 (2007).

    Google Scholar 

  12. N. A. Farooqui, A. Dey, G. N. Singh, et al., Asian J. Pharmaceut. Clin. Res., 7, 235 – 240 (2014).

    Google Scholar 

  13. K. R. Brain, B. B. Thapa, J. Chromatogr., 258, 183 – 188 (1983).

    Article  CAS  Google Scholar 

  14. S. Muralidhar, S. Shashidhara, P. E. Rajasekharan, et al., Int. J. Drug Developm. Res., 2, 676 – 680 (2010).

    CAS  Google Scholar 

  15. K. Dhalwal, V. M. Shinde, K. R. Mahadik, J. Med. Plants Res., 4, 1289 – 1296 (2010).

    CAS  Google Scholar 

  16. M. Garg, E. T. Tamboli, P. Rabea, et al., Pharmacognocy Magazine, 10, 198 – 205 (2014).

    Article  Google Scholar 

  17. B. Avula, S. Begum, S. Ahmed, et al., Die Pharmazie - An Int. J. Pharm. Sci., 63, 20 – 22 (2008).

    CAS  Google Scholar 

  18. Indian Pharmacopoeia, Government of India, Ministry of Health and Family Welfare, Delhi, India, (1996).

  19. A. O. A. C. Official Methods of Analysis. Association of Official Analytical chemists. EUA, (2000).

  20. S. Y. Gabhe, P. A. Tatke, R. K. Punjabi, Int. J. Pharmacognosy Phytochem. Res., 10, 350 – 354 (2018).

    Google Scholar 

  21. B. R. Keesara, R. K. Jat, Int. J. Res. Developm. Pharm. & Life Sci., 6, 2590 – 2596 (2017).

  22. ICH Topic Q 1 A(R2) Stability Testing of new Drug Substances and Products (2003).

  23. ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2 (R1) (2005).

Download references

Author information

Authors and Affiliations

  1. Department of Quality Assurance Techniques, Bharati Vidyapeeth (Deemed To Be University), Poona College of Pharmacy, Erandwane, Pune, 411 038, India

    S. J. Satpute

  2. Department of Pharmaceutical Chemistry, Bharati Vidyapeeth (Deemed To Be University), Poona College of Pharmacy, Erandwane, Pune, 411 038, India

    S. S. Yadav & S. S. Chopade

Authors
  1. S. J. Satpute
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. S. Yadav
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. S. Chopade
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. S. Yadav.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Satpute, S.J., Yadav, S.S. & Chopade, S.S. HPTLC Method Development for Estimation of Vasicine in Herbal Extract and its Marketed Formulations. Pharm Chem J 57, 1284–1289 (2023). https://doi.org/10.1007/s11094-024-03036-x

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11094-024-03036-x

Keywords

Search

Navigation