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Anti-Inflammatory, Wound Healing, and Hepatoprotective Effects of Ethanol Extract of Rice Bran in Rodent Models

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Pharmaceutical Chemistry Journal Aims and scope

Rice bran is an essential co-product in rice meals with many biological activities. This study was aimed at evaluating the anti-inflammatory, wound healing, and hepatoprotective effects of ethanol extract of rice bran in vivo. The anti-inflammatory and hepatoprotective studies were performed in rats, and the wound-healing study was conducted in mice. Carrageenan and carbon tetrachloride were used to induce inflammation and hepatotoxicity respectively. The wound-healing activity of rice bran extract (RBE) was tested by creating a circular wound using a biopsy punch. For all the studies, animals were treated with RBE at a dose rate of 100 mg/kg and 200 mg/kg body weight (bwt). It was observed that RBE dose-dependently reduced the carrageenan-induced paw edema and wound-closing time (9–10 days) compared with that of control rats (12 days). Histological examination of the inflamed rat paw tissue showed massive infiltration of inflammatory cells, which was significantly reduced by RBE treatment. Intra-peritoneal injection of CCl4 increased the serum AST and ALT levels, which were significantly (p < 0.05) decreased by RBE treatment in a dose-dependent manner. The histological section of the liver tissue showed severe hepatic cell damage in CCl4-injected animals, which were distinctly regenerated by RBE treatment (200 mg/kg bwt). These results demonstrated that RBE has potent anti-inflammatory, wound-healing, and hepatoprotective effects in the experimental animal.

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Acknowledgments

This work was supported by the special allocation project for the year 2021–2022 under the Ministry of Science and Technology (MoST), Bangladesh (No. 39.00.0000.009.06.009.14.019.21/BS–41).

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Authors and Affiliations

  1. Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Pritam Saha, Purba Islam, Kazi Rafiq & Md.Zahorul Islam

  2. Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Rafiqul Islam

  3. Department of Pharmacology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan

    Atsushi Miyamoto

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  1. Pritam Saha
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  2. Purba Islam
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Correspondence to Md.Zahorul Islam.

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Saha, P., Islam, P., Rafiq, K. et al. Anti-Inflammatory, Wound Healing, and Hepatoprotective Effects of Ethanol Extract of Rice Bran in Rodent Models. Pharm Chem J 57, 1410–1417 (2023). https://doi.org/10.1007/s11094-023-03004-x

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  • DOI: https://doi.org/10.1007/s11094-023-03004-x

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