Skip to main content
SpringerLink
Log in

Development and Validation of a Method for Assay of Porphyrins and Their Precursors by HPLC with UV Detection in Human Plasma

  • Published:
Pharmaceutical Chemistry Journal Aims and scope

Porphyrinogenic drugs can cause changes in heme metabolism, leading to increases in porphyrin levels in biological fluids and exacerbation of the course of porphyrias. The sites at which porphyrins and their precursors accumulate determine the course and clinical picture of porphyrias, i.e., liver (hepatic types) and bone marrow (erythropoietic types). Porphyria biomarkers are found in urine, feces, and plasma. A reverse phase HPLC technique with UV detection was validated. Qualitative and quantitative assessment of the composition of heme precursors (nine markers + internal standard) in blood plasma was carried out on a Waters Acquity UPLC H-CLASS system with a diode array detector.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. D. A. Kil’dyushkin, A. E. Petukhov, E. A. Litvin, Farmakokin. Farmakodin., No. 2, 36 – 45 (2022).

  2. V. Poulos, W. H. Lockwood, Int. J. Biochem., 12(5) – 6, 959 – 961 (1980).

  3. M. Danton and C. K. Lim, Biomed. Chromatogr., 20(6 – 7), 612 – 621 (2006).

    Article  CAS  PubMed  Google Scholar 

  4. K. Sorte, K. Palandurkar, M. Goyal, et al., J. Clin. Diagn. Res., 3, 2031 – 2035 (2010).

    Google Scholar 

  5. M. Biesaga, K. Pyrzynska, M. Trojanowicz, Talanta, 51, 209 – 224 (2000).

    Article  CAS  PubMed  Google Scholar 

  6. W. Huang, Q. Liu, E.-Y. Zhu, et al., Talanta, 82, 1516 – 1520 (2010).

    Article  CAS  PubMed  Google Scholar 

  7. E. DiPierro, M. DeCanio, R. Mercadante, et al., Diagnostics, 11, 1343 (2021).

    Article  CAS  Google Scholar 

  8. L. Chularojanamontri, C. Tuchinda, C. Srisawat, et al., J. Med. Assoc. Thai., 91(12), 1915 – 1919 (2008).

    PubMed  Google Scholar 

  9. A. Nimesh, V. Agarwal, S. Garg, and M. Mehndiratta, Ind. J. Med. Biochem., 21(2), 136 – 141 (2017).

    Article  Google Scholar 

  10. E. Magi, C. Ianni, P. Rivaro, et al., J. Chromatogr. A, 905, No. 1 – 2, 141 – 149 (2001).

    Article  CAS  PubMed  Google Scholar 

  11. M. Tahoun, C. T. Gee, V. E. McCoy, et al., RSC Adv., 11, 7552 – 7563 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. H. D. Meyer, K. Jacob, W. Vogt, in: XI Int. Congr. of Clin. Chem., 2nd edition. E. Kaiser, F. Gabl, M. M. Müller, P. M. Bayer (eds.) De Gruyter, Berlin, Germany; Boston, MA, USA; Vienna, Austria (2019), pp. 1001 – 1006.

  13. D. T. Zelt, J. A. Owen, G. S. Marks, J. Chromatogr., 189(2), 209 – 116 (1980).

    Article  CAS  PubMed  Google Scholar 

  14. H. de Verneuil, S. Sassa, A. Kappas, J. Biol. Chem., 258(4), 2454 – 2460 (1983).

    Article  PubMed  Google Scholar 

  15. J. Fyrestam, N. Bjurshammar, E. Paulsson, et al., Anal. Bioanal. Chem., 407, 7013 – 7023 (2015).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. C. Taylor, L. K. Duffy, F. G. Plumley, and R. T. Bowyer, Environ. Res., 84(1), 56 – 63 (2000).

    Article  CAS  PubMed  Google Scholar 

  17. Y. Hur, S. Tae, Y. Koh, et al., Mass Spectrometry Lett., 5(2), 42 – 48 (2014).

    Article  CAS  Google Scholar 

  18. J. S. Woods and P. L. Simmonds, Curr. Protoc. Toxicol., Chapter 8, Unit 8.9 (2001); doi: https://doi.org/10.1002/0471140856.tx0809s07.

  19. I. San Juan, C. Bruzzone, M. Bizkarguenaga, et al., Br. J. Haematol., 190(5), e265-e267 (2020).

  20. R. Mateo, G. Castells, A. J. Green, et al., J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci., 810(2), 305 – 311 (2004).

  21. J. To-Figueras, J. D. Phillips, J. M. Gonzalez-López, et al., Br. J. Dermatol., 165(3), 499 – 505 (2011).

    CAS  PubMed  Google Scholar 

  22. ICH Harmonized Tripartite Guidelines, ICH Topic Q2 (R1) “Validation of analytical procedures: Text and methodology, ICH, Geneva (1995).

    Google Scholar 

  23. FDA Guidance Documents “Analytical Procedures and Methods Validation for Drugs and Biologics” FDA(July, 2015).

  24. OFS. 1.1.0012.15 “Validation of analytical methods,” in: State Pharmacopeia of the Russian Federation [in Russian], XIV edition (2018).

  25. Guidelines on the Validation of Analytical Methods for Testing Medicinal Products [in Russian], Approved by decision of the Collegium of Countries of the Eurasian Economic Commission, July 17, 2018, No. 113 (2018).

  26. Guidance on Validation of Drug Analysis Methods [in Russian], Russian Federation Ministry of Health and Social Development, Moscow (2007).

  27. Pharmacopeia (USP-31), General Chapters: Validation of compendial methods.

  28. ICH Q2B, International Conference on Harmonisation, Validation of analytical procedures, Methodology (2002).

  29. Pharmacopeia of the Eurasian Economic Union, 2.1. General pharmacopeia article OFS 5.31. Validation of analytical methods.

Download references

Author information

Authors and Affiliations

  1. I. M. Sechenov First Moscow State Medical University, Russian Ministry of Health (Sechenov University), Building 2, 8 Trubetskaya Stret, 119991, Moscow, Russia

    D. A. Kil’dyushkin, A. M. Sukhanova, T. V. Korochkina, V. I. Gegechkori & A. E. Petukhov

  2. Dmitry Rogachev National Medical Research Center for Pediatric Hematology, Oncology, and Immunology, Russian Ministry of Health, 1 Samory Mashela Street, 117997, Moscow, Russia

    E. A. Litvin

Authors
  1. D. A. Kil’dyushkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. A. Litvin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. Sukhanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. V. Korochkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. I. Gegechkori
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. E. Petukhov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. A. Kil’dyushkin.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 57, No. 7, pp. 55 – 60, July, 2023

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kil’dyushkin, D.A., Litvin, E.A., Sukhanova, A.M. et al. Development and Validation of a Method for Assay of Porphyrins and Their Precursors by HPLC with UV Detection in Human Plasma. Pharm Chem J 57, 1089–1094 (2023). https://doi.org/10.1007/s11094-023-02988-w

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11094-023-02988-w

Keywords

Search

Navigation