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Studies of the Possibility of Using Renal Biomarkers to Assess Drug Nephrotoxicity in an RPTEC/TERT1 Cell Line Model

  • MOLECULAR BIOLOGICAL PROBLEMS OF DRUG DESIGN AND MECHANISM OF DRUG ACTION
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Pharmaceutical Chemistry Journal Aims and scope

We report here studies of the possibility of using contemporary biomarkers of in vivo kidney damage - cystatin C and clusterin – in the RPTEC/TERT1 cell line model. The drugs used as nephrotoxic agents were cisplatin (cis-[Pt(NH3)2Cl2]) and vancomycin. Biomarker protein concentrations were measured by enzyme immunoassay. A sharp increase in the clusterin concentration was demonstrated during the first 4 h of incubation with vancomycin, after which the clusterin concentration returned to normal. The cystatin C concentration increased gradually throughout the entire incubation period on exposure to both cisplatin and vancomycin. These results demonstrate that clusterin and cystatin C could be used as in vitro biomarkers to assess drug-induced nephrotoxicity in RPTEC/TERT1/TERT1 cells.

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Funding

This work was carried out within the framework of the state contract with the Scientific Center for Expert Evaluation of Medicinal Products, Russian Ministry of Health, No. 056-00005-21-00 for applied scientific research (State account No. NIR 121022400082-4).

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Authors and Affiliations

  1. Scientific Center for Expert Evaluation of Medicinal Products, Building 2, 8 Petrovskii Boulevard, Moscow, Russia

    V. A. Evteev, I. S. Semenova, N. D. Bunyatyan & A. B. Prokof’ev

  2. I. M. Sechenov First Moscow State Medical University, Russian Ministry of Health (Sechenov University), Building 2, 8 Trubetskaya Street, Moscow, Russia

    N. D. Bunyatyan & A. B. Prokof’ev

Authors
  1. V. A. Evteev
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  2. I. S. Semenova
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  3. N. D. Bunyatyan
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  4. A. B. Prokof’ev
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Corresponding author

Correspondence to V. A. Evteev.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 57, No. 7, pp. 3 – 6, July, 2023.

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Evteev, V.A., Semenova, I.S., Bunyatyan, N.D. et al. Studies of the Possibility of Using Renal Biomarkers to Assess Drug Nephrotoxicity in an RPTEC/TERT1 Cell Line Model. Pharm Chem J 57, 953–956 (2023). https://doi.org/10.1007/s11094-023-02970-6

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  • DOI: https://doi.org/10.1007/s11094-023-02970-6

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