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IN-VITRO Assessment of Anti-Cholinesterase, Anti-Lipase, Antioxidant Activities and Photoprotective Effect of Algerian Fagonia bruguieri DC Extracts

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Pharmaceutical Chemistry Journal Aims and scope

This is the first study aimed to investigate the anti-cholinesterase (anti-Alzheimer), anti-lipase, antioxidant and photoprotective effects of various organic extracts prepared from the aerial parts of Fagonia bruguieri DC species endemic to southern Algeria. The research was also aimed to determine the total phenolics and flavonoid contents and evaluate in vitro pharmacological activities of these extracts by means of spectrophoto-metric analysis. The in vitro antioxidant properties of F. bruguieri were examined by DPPH radical scavenging, -β-carotene bleaching, ferric reducing power, cupric reducing antioxidant capacity (CUPRAC), free galvinoxyl radical (GOR), and O-phenanthroline assays. The anti-Alzheimer action was characterized by inhibiting of the activity of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The anti-lipase test was used to evaluate the inhibitory capacity against pancreatic lipase. In addition, the photoprotective effect was estimated by determining in vitro sun protection factor (SPF) of F. bruguieri extracts. The results showed that polar extracts exhibited excellent antioxidant activity in various systems, with the best performance of butanol extract. The butanol extract contains a significant amount of phenolics and flavonoids (444.49 ± 0.84 μg GAE/mg and 266.45 ± 0.44 μg QE/mg, respectively). The petroleum ether extract shows a potent AChE and BChE activity with IC50 = 27.41 ± 1.03 μg/mL and 46.14 ± 0.78 μg/mL, respectively. Concerning the anti-lipase effect, dichloromethane extract showed a strong inhibition against the lipase enzyme. In addition, F. bruguieri exhibited high photoprotective activity for absorbing UV radiations with SPF index ranging from 11.25 ± 0.1 to 43.86 ± 0.12. These findings suggest that F. bruguieri extracts provide a valuable source of antioxidants that can be explored in multiple therapeutic remedies and can be used for the prevention of Alzheimer’s disease and as anti-obesity agent. Extracts of F. bruguieri DC can also be used to treat skin conditions as cosmetic formulations and in the pharmaceutical industry.

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Authors and Affiliations

  1. Laboratoire de valorisation et promotion des ressources sahariennes (VPRS), Département de Chimie, Faculté des mathématiques et des Sciences de la Matière, Université de Ouargla, Ouargla, Algeria

    Wafa Zahnit, Ouanissa Smara, Lazhar Bechki & Messaouda Dekmouche

  2. Centre de Recherche en Biotechnologie (C.R.B.T) Constantine, Constantine, Algeria

    Chawki Bensouici

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  1. Wafa Zahnit
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Correspondence to Wafa Zahnit.

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Zahnit, W., Smara, O., Bechki, L. et al. IN-VITRO Assessment of Anti-Cholinesterase, Anti-Lipase, Antioxidant Activities and Photoprotective Effect of Algerian Fagonia bruguieri DC Extracts. Pharm Chem J 57, 89–100 (2023). https://doi.org/10.1007/s11094-023-02855-8

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