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Effect of Tin(II) Chloride Amount on 99mTc-Labeling of One 6-Hydrazinonicotinamide-Conjugated Peptide in the Presence of Tricine/Nicotinic Acid

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Pharmaceutical Chemistry Journal Aims and scope

The use of tin(II) chloride in amount above its required mole ratio as reducing agent can lead to instability of peptide-based radiopharmaceuticals. In this work, we optimize the radiolabeling conditions, especially tin(II) chloride amount for 99mTc-labeling of one 6-hydrazinonicotinamide (HYNIC) conjugated peptide in the presence of tricine and nicotinic acid (NA). Implementation of this procedure leads to 99mTc-labeling of this HYNIC-conjugated peptide in high radiochemical yield (>95%). By comparison, our experimental results can provide an efficacious strategy for 99mTc-labeling of HYNIC-conjugated biomolecules in the presence of tricine/NA. The resulting 99mTc-labeled peptide, without the need for any chemical modification and pretreatment, shows good stability in saline and human serum. This work can provide a new perspective for 99mTc-labeling of HYNIC-conjugated biomolecules.

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Authors and Affiliations

  1. Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

    Sajad Kaihani & Nourollah Sadeghzadeh

  2. Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

    Sajad Kaihani

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  1. Sajad Kaihani
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  2. Nourollah Sadeghzadeh
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Correspondence to Nourollah Sadeghzadeh.

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Kaihani, S., Sadeghzadeh, N. Effect of Tin(II) Chloride Amount on 99mTc-Labeling of One 6-Hydrazinonicotinamide-Conjugated Peptide in the Presence of Tricine/Nicotinic Acid. Pharm Chem J 56, 1075–1081 (2022). https://doi.org/10.1007/s11094-022-02756-2

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  • DOI: https://doi.org/10.1007/s11094-022-02756-2

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