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Comparison of the Independent and Combined Effects of Aerobic Training and Ozone Therapy on Selected Heart Variables in Rats with Osteoarthritis

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Pharmaceutical Chemistry Journal Aims and scope

There are shreds of evidence that osteoarthritis is a risk factor for cardiovascular disease. Regular exercise and ozone therapy independently can prevent such diseases; however, their combined effect was not investigated until now. The current study was aimed to compare the independent and combined effects of aerobic training and ozone therapy on mitochondrial fission 1 protein (FIS1) and mitofusin 2 (MFN2) gene expressions, KI67 protein expression, and percentage collagen deposition in the heart tissue of rats with osteoarthritis. Thirty rats were randomly divided into five groups (six per group), named healthy control, osteoarthritis control, osteoarthritis training, osteoarthritis ozone, and osteoarthritis training + ozone. The interventions were performed according to the group name (aerobic training: run on the treadmill five sessions/week, 30 – 50 min/session for 8 weeks; ozone therapy: 20 μg/mL, once/week, for three weeks). Rat heart tissue was collected 48 h after the last intervention. Data were analyzed using two-way ANOVA and P < 0.05 was considered as significant effect. The results showed that osteoarthritis intervention significantly increased the expression of FIS-1 and MFN-2 genes as well as the percentage of collagen degradation in heart tissue. Osteoarthritis also decreased expression of the heart KI67 protein. Aerobic training and ozone therapy independently positively affect mentioned variables; however, the synergic effect was seen just for the percentage of collagen degradation. In conclusion, the present study confirms effectiveness of the aerobic exercise and ozone therapy concerning research variables and shows that combination of the exercise and ozone therapy had a greater effect than using either alone in improving collagen degradation.

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Authors and Affiliations

  1. Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

    A. Zandi & H. Matinhomaee

  2. Department of Physical Education and Sports Science, North Tehran Branch, Islamic Azad University, Tehran, Iran

    L. Moradi

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  1. A. Zandi
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  2. H. Matinhomaee
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  3. L. Moradi
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Correspondence to H. Matinhomaee.

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Zandi, A., Matinhomaee, H. & Moradi, L. Comparison of the Independent and Combined Effects of Aerobic Training and Ozone Therapy on Selected Heart Variables in Rats with Osteoarthritis. Pharm Chem J 56, 1033–1040 (2022). https://doi.org/10.1007/s11094-022-02748-2

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  • DOI: https://doi.org/10.1007/s11094-022-02748-2

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