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Physicochemical and Bioassay Directed GC-MS Analyses of Chloroform Extract of Pteris cretica L.

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Pharmaceutical Chemistry Journal Aims and scope

This study was aimed to explore the potential of Pteris cretica L. to be used as herbal remedy. The P. cretica dried plant powder was subjected to a standard physicochemical (proximate analysis) protocol, which showed promising results and was consistent with the WHO recommended ranges that are required to use the plant as a remedy. To identify the bioactive chemical constituents of chloroform extract, the extract was subjected to a series of column chromatography and p-HPLC through the antibacterial and antioxidant bioassays guidance. The two most bioactive sub-fractions of chloroform extract were subjected to GC-MS analysis which revealed the presence of seventeen compounds, the major constituents being eicosane (16.8%), hexadecane (14.1%), methyl hexadecanoate (11.2%), 3,3,8-trimethyldecane (9.1%), 2,6,10-trimethyldodecane (7.8%), tricosane (5.1%) and tetradecane (3.86%). Proximate analysis results show that P. cretica plant is good for herbal remedy, and the bioassay results on chloroform fractions and sub-fractions show that this plant has significant antibacterial and antioxidant potentials. To use this plant as herbal remedy, there is need in further exploration.

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Acknowledgments

The authors express their gratitude to the Faculty of Pharmacy, University of Lahore, Pakistan, and the International Center for Chemical and Biological Sciences (ICCBS), H. E. J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan for providing necessary research facilities.

Conflict of Interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan

    Farooq Saleem & Muhammad Azeem

  2. Department of Chemistry, Division of Science and Technology, University of Education, Township, Lahore, Pakistan

    Rashad Mehmood

  3. Akhtar Saeed College of Pharmaceutical Sciences, Lahore, Pakistan

    Tahir Javed Khan

  4. Imran Idress College of Pharmacy, Daska Road 3 km, Sialkot, Pakistan

    Saeed-ul Hassen

  5. Department of Chemistry, Sardar Bahadur Khan Women University, Quetta, Pakistan

    Saima Maher

  6. Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

    Sarfraz Ahmad

  7. Department of Chemistry, School of Computer, Mathematical and Natural Sciences, Morgan State University, Baltimore, MD, 21251, USA

    Shagufta Perveen

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  1. Farooq Saleem
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  2. Rashad Mehmood
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  3. Tahir Javed Khan
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  7. Shagufta Perveen
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  8. Muhammad Azeem
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Correspondence to Farooq Saleem.

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Saleem, F., Mehmood, R., Khan, T.J. et al. Physicochemical and Bioassay Directed GC-MS Analyses of Chloroform Extract of Pteris cretica L.. Pharm Chem J 56, 387–395 (2022). https://doi.org/10.1007/s11094-022-02648-5

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  • DOI: https://doi.org/10.1007/s11094-022-02648-5

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