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Antioxidant and Genoprotective Properties of α-Lipoic (Thioctic) Acid in Blood Serum Under X-Ray Exposure

  • MOLECULAR-BIOLOGICAL PROBLEMS OF DRUG DESIGN AND MECHANISM OF DRUG ACTION
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Pharmaceutical Chemistry Journal Aims and scope

α-Lipoic (thioctic) acid (ALA), which is used as a drug for the treatment of diabetic and alcoholic neuropathy and several other diseases, was found to be an effective antioxidant that effectively neutralized the products of water radiolysis and reduced oxidative damage to DNA and proteins caused by x-ray exposure. ALA had different effects on the formation and decay of long-lived reactive species of albumin and globulin in blood serum. This process was accompanied by the generation of hydrogen peroxide and prolongation of post-exposure oxidative stress. ALA in the concentration range 0.05 – 0.4 nM eliminated oxidative damage of DNA in vitro although the efficiency decreased with increasing concentration. ALA also exhibited genoprotective and radiomitigatory properties in vivo by reducing the formation of micronuclei in polychromatophilic erythrocytes of red bone marrow in mice after irradiation. The obtained results and available literature data indicate that ALA in the effective pharmaceutical window is a promising therapeutic radiomitigator capable of preventing harmful consequences caused by the disturbance of redox homeostasis due to radiation-induced oxidative stress.

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References

  1. E. I. Azzam, J. P. Jay-Gerin, and D. Pain, Cancer Lett., 327, No. 1 – 2, 48 – 60 (2012).

  2. S. V. Gudkov, N. R. Popova, and V. I. Bruskov, Biofizika, 60(4), 801 – 811 (2015).

    CAS  PubMed  Google Scholar 

  3. G. E. Hanumakumar, M. Balaji, and R. S. Karunakaran, Int. J. Pharm. Sci. Res., 9(7), 2656 – 2668 (2018).

    CAS  Google Scholar 

  4. M. V. Vasin and I. B. Ushakov, Usp. Sovrem. Biol., 139(3), 235 – 253 (2019).

    Google Scholar 

  5. M. V. Vasin and I. B. Ushakov, Usp. Sovrem. Biol., 140(1), 3 – 18 (2020).

    Google Scholar 

  6. L. Yu. Morgunov, Med. Sovet, No. 17, 90 – 94 (2014).

  7. O. V. Kurushina, A. E. Barulin, and E. P. Chernovolenko, Med. Sovet, No. 1, 58 – 63 (2019).

  8. K. P. Shay, R. F. Moreau, E. J. Smith, et al., Biochim. Biophys. Acta, 1790(10), 1149 – 1160 (2009).

    Article  CAS  Google Scholar 

  9. C. Waslo, D. Bourdette, N. Gray, et al., Curr. Treat. Options Neurol., 21(6), 26 (2019).

    Article  Google Scholar 

  10. B. Diesel, S. Kulhanek-Heinze, M. Holtje, et al., Biochemistry, 46(8), 2146 – 2155 (2007).

    Article  CAS  Google Scholar 

  11. K. P. Shay, A. J. Michels, W. Li, et al., Biochim. Biophys. Acta, 1823(6), 1102 – 1109 (2012).

    Article  CAS  Google Scholar 

  12. S. Morkunaite, V. V. Teplova, and N. E. Saris, IUBMB Life, 49(3), 211 – 216 (2000).

    Article  CAS  Google Scholar 

  13. J. H. Suh, B.-Z. Zhu, E. deSzoeke, et al., Redox Rep., 9(1), 57 – 61 (2004).

    Article  CAS  Google Scholar 

  14. S. V. Gudkov, O. E. Karp, S. A. Garmash, et al., Biofizika, 57(1), 5 – 13 (2012).

    CAS  PubMed  Google Scholar 

  15. V. I. Bruskov, O. E. Karp, S. A. Garmash, et al., Free Radical Res., 46(10), 1280 – 1290 (2012).

    Article  CAS  Google Scholar 

  16. I. N. Shtarkman, S. V. Gudkov, A. V. Chernikov, and V. I. Bruskov, Biokhimiya, 73(4), 576 – 586 (2008).

    Google Scholar 

  17. V. E. Ivanov, A. M. Usacheva, A. V. Chernikov, et al., J. Photochem. Photobiol. B, 176, 36 – 43 (2017).

    Article  CAS  Google Scholar 

  18. V. E. Ivanov, A. V. Chernikov, S. V. Gudkov, and V. I. Bruskov, Biofizika, 63(5), 873 – 879 (2018).

    Google Scholar 

  19. E. E. Karmanova, S. A. Abdullaev, V. E. Ivanov, et al., IOP Conf. Ser.: Mater. Sci. Eng., 487, 012023 (2019).

  20. V. I. Bruskov, A. I. Gaziev, L. V. Malakhova, et al., Biokhimiya, 61(4), 737 – 745 (1996).

    CAS  Google Scholar 

  21. A. Vral, M. Fenech, and H. Thierens, Mutagenesis, 26(1), 11 – 17 (2011).

    Article  CAS  Google Scholar 

  22. E. E. Karmanova, A. V. Chernikov, A. M. Usacheva, and V. I. Bruskov, Khim.-farm. Zh., 54(7), 10 – 14 (2020); Pharm. Chem. J., 54(7), 673 – 677 (2020).

  23. V. I. Bruskov, A. V. Chernikov, V. E. Ivanov, et al., Phys. Wave Phenom., 28(2), 91 – 101 (2020).

    Article  Google Scholar 

  24. M. Roche, P. Rondeau, N. R. Singh, et al., FEBS Lett., 582, 1783 – 1787 (2008).

    Article  CAS  Google Scholar 

  25. A. M. Usacheva, A. V. Chernikov, E. E. Karmanova, and V. I. Bruskov, Khim.-farm. Zh., 55(11), 9 – 17 (2021); Pharm. Chem. J., 55(11), 1138 – 1146 (2022).

  26. A. Piechota-Polanczyk, M. Zielinska, D. Piekielny, and J. Fichna, Biomed. Pharmacother., 84, 470 – 475 (2016).

    Article  CAS  Google Scholar 

  27. F. Bhatti, R. W. Mankhey, L. Asico, et al., Kidney Int., 67(4), 1371 – 1380 (2005).

    Article  CAS  Google Scholar 

  28. S. L. Brown, A. Kolozsvary, J. Liu, et al., Radiat Res., 173(4), 462 – 468 (2010).

    Article  CAS  Google Scholar 

  29. L. Ramachandran, C. Krishnan, and K. Nair, Mutat. Res., Genet. Toxicol. Environ. Mutagen., 724, 52 – 58 (2011).

  30. S. H. Ryu, E. Y. Park, S. Kwak, et al., Oncotarget, 7(13), 15554 – 15565 (2016).

    Article  Google Scholar 

  31. R. S. Said, A. Mohamed, and D. H. Kassem, Toxicology, 442, 152536 (2020).

Download references

Acknowledgments

The work was performed on a State Task for IGEB, RAS (Topic No. 05, Direction 59).

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Correspondence to E. E. Karmanova.

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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 56, No. 3, pp. 3 – 7, March, 2022.

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Karmanova, E.E., Chernikov, A.V., Ivanov, V.E. et al. Antioxidant and Genoprotective Properties of α-Lipoic (Thioctic) Acid in Blood Serum Under X-Ray Exposure. Pharm Chem J 56, 295–299 (2022). https://doi.org/10.1007/s11094-022-02641-y

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  • DOI: https://doi.org/10.1007/s11094-022-02641-y

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