Skip to main content
SpringerLink
Log in

The Influence of Bovine Serum Albumin Modified Titanium Dioxide Nanoparticles on Myoblast Cytotoxicity

  • Published:
Pharmaceutical Chemistry Journal Aims and scope

Titanium dioxide nanoparticles (TiO2NPs) are utilised as a key constituent in many biopharmaceuticals and food products. This is mainly attributed to their favorable chemical properties which enhance their biocompatibility. However, alteration of the physicochemical properties in various matrices remains unexplored. The present study aims to determine if TiO2NPs modified by bovine serum albumin (BSA) have the ability to influence cytotoxicity in C2C12 myoblast cells. The physicochemical properties of TiO2NPs were altered using BSA in a concentration range of 0.3 – 1.5 mg/mL and characterised using nanoparticle (NP) tracking analysis and transmission electron microscopy. Results show that cytotoxicity is enhanced at a BSA concentration of 0.8 mg/mL, which agrees with reduced particle agglomeration and favorable zeta potential. This proves that the state of TiO2 NPs agglomeration directly affects cytotoxicity which can be due to internalization of the smaller nanoparticle (NP) aggregates. These findings suggest that in vitro cytotoxicity assays incorporate the physicochemical characteristics of NPs in relation to the specific cell model used and colloidal state of the substance being tested.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.

Similar content being viewed by others

References

  1. M. Hedenborg, Int. Arch. Occup. Environ. Health, 61, 1 – 6 (1988).

    Article  CAS  Google Scholar 

  2. Y. Duan, J. Liu, L. Ma, et al., Biomaterials, 31, 894 – 899 (2010).

    Article  CAS  Google Scholar 

  3. J. J. Jacobs, A. K. Skipor, J. Black, et al., J. Bone Joint Surg. Am., 73, 1475 – 1486 (1991).

    Article  CAS  Google Scholar 

  4. R. Wolf, H. Matz, E. Orion, and J. Lipozencic, Acta Dermatovenerol. Croat., 11, 158 – 162 (2003).

    PubMed  Google Scholar 

  5. D. B. Warheit, T. R. Webb, C. M. Sayes, et al., Toxicol. Sci., 91, 227 – 236 (2006).

    Article  CAS  Google Scholar 

  6. I. Iavicoli, V. Leso, and A. Bergamaschi, J. Nanomater., 2012, 5 (2012).

    Google Scholar 

  7. R. D. Handy and B. J. Shaw, Health. Risk Soc., 9, 125 – 144 (2007).

    Article  Google Scholar 

  8. M.-P. Ling, C.-P. Chio, W.-C. Chou, et al., Environ. Sci. Pollut. Res., 18, 877 – 889 (2011).

    Article  CAS  Google Scholar 

  9. N. Li and A. E. Nel, J. Occup. Environ. Med. Coll. Occup. Environ. Med., 53, S74 (2011).

    Article  CAS  Google Scholar 

  10. R. A. Yokel and R. C. Macphail, J. Occup. Med. Toxicol., 6, 7 (2011).

    Article  CAS  Google Scholar 

  11. A. Nel, T. Xia, L. Mädler, and N. Li, Science, 311, 622 – 627 (2006).

    Article  CAS  Google Scholar 

  12. P. Baveye and M. Laba, Environ. Health Perspect., 116, A152–A152 (2008).

    Article  Google Scholar 

  13. K. M. Waters, L. M. Masiello, R. C. Zangar, et al., Toxicol. Sci., 107, 553 – 569 (2008).

    Article  CAS  Google Scholar 

  14. J. Okuda-Shimazaki, S. Takaku, K. Kanehira, et al., Int. J. Mol. Sci., 11, 2383 – 2392 (2010).

    Article  CAS  Google Scholar 

  15. B. D. Chithrani and W. C. W. Chan, Nano Lett., 7, 1542 – 1550 (2007).

    Article  CAS  Google Scholar 

  16. K. W. Powers, S. C. Brown, V. B. Krishna, et al., Toxicol. Sci., 90, 296 – 303 (2006).

    Article  CAS  Google Scholar 

  17. F. Blank, B. M. Rothen-Rutishauser, S. Schurch, and P. Gehr, J. Aerosol Med., 19, 392 – 405 (2006).

    Article  Google Scholar 

  18. M. Geiser, B. Rothen-Rutishauser, N. Kapp, et al., Environ. Health Perspect., 113, 1555 – 1560 (2005).

    Article  Google Scholar 

  19. V. H. Grassian, A. Adamcakova-Dodd, J. M. Pettibone, et al., Nanotoxicology, 1, 211 – 226 (2007).

    Article  CAS  Google Scholar 

  20. D. B. Warheit, T. R. Webb, K. L. Reed, et al., Toxicology, 230, 90 – 104 (2007).

    Article  CAS  Google Scholar 

  21. H. L. Karlsson, J. Gustafsson, P. Cronholm, and L. Müller, Toxicol. Lett., 188, 112 – 118 (2009).

    Article  CAS  Google Scholar 

  22. C. Bantz, O. Koshkina, T. Lang, et al., Beilstein J. Nanotechnol., 5, 1774 – 1786 (2014).

    Article  CAS  Google Scholar 

  23. S. S Moni, M. M Safhi, and B. B Barik, Curr. Pharm. Biotechnol., 14, 1242 – 1249 (2013).

    Article  CAS  Google Scholar 

  24. A. Mohammadipour, A. Fazel, H. Haghir, et al., Environ. Toxicol Pharmacol., 37, 617 – 625 (2014).

    Article  CAS  Google Scholar 

  25. K. S. Hougaard, L. Campagnolo, P. Chavatte-Palmer, et al., Reprod. Toxicol., 56, 118 – 140 (2015).

    Article  CAS  Google Scholar 

  26. R. Tantra, J. Tompkins, and P. Quincey, Colloids Surfaces B Biointerfaces, 75, 275 – 281 (2010).

    Article  CAS  Google Scholar 

  27. E. J. Cho, H. Holback, K. C. Liu, et al., Mol. Pharm., 10, 2093 – 2110 (2013).

    Article  CAS  Google Scholar 

  28. H. Zuo, Z. Gu, H. Cooper, and Z. P. Xu, J. Colloid Interface Sci., 459, 10 – 16 (2015).

    Article  CAS  Google Scholar 

  29. P. Bihari, M. Vippola, S. Schultes, et al., Part. Fibre Toxicol., 5, 14 (2008).

    Article  CAS  Google Scholar 

  30. Z. Ji, X. Jin, S. George, et al., Environ. Sci. Technol., 44, 7309 – 7314 (2010).

    Article  CAS  Google Scholar 

  31. D. Mahl, C. Greulich, W. Meyer-Zaika, et al., J. Mater. Chem., 20, 6176 – 6181 (2010).

    Article  CAS  Google Scholar 

  32. H. T. R. Wiogo, M. Lim, V. Bulmus, et al., Langmuir, 27, 843 – 850 (2010).

    Article  CAS  Google Scholar 

  33. S. Bhattacharjee, L. H. de Haan, N. M. Evers, et al., Part Fibre Toxicol, 7, 25 (2010).

    Article  CAS  Google Scholar 

  34. L. Yildirimer, N. T. K. Thanh, M. Loizidou, and A. M. Seifalian, Nano Today, 6, 585 – 607 (2011).

    Article  CAS  Google Scholar 

  35. A. Albanese and W. C. W. Chan, ACS Nano, 5, 5478 – 5489 (2011).

    Article  CAS  Google Scholar 

  36. A. Panariti, G. Miserocchi, and I. Rivolta, Nanotechnol. Sci. Appl., 5, 87 (2012).

    PubMed  PubMed Central  CAS  Google Scholar 

  37. C. M. Hussain and B. Kharisov, Advanced Environmental Analysis: Applications of Nanomaterials, London: Royal Society of Chemistry (2016).

    Book  Google Scholar 

  38. A. Lankoff, W. J. Sandberg, A. Wegierek-Ciuk, et al., Toxicol. Lett., 208, 197 – 213 (2012).

    Article  CAS  Google Scholar 

  39. P. Pillay and I. Mackraj, Preliminary Characterization of Exosomes from Maternal Circulation, University of Kwa-Zulu Natal, Durban: Kwa-Zulu Natal, South Africa (2016).

    Google Scholar 

  40. B. Michen, C. Geers, D. Vanhecke, et al., Sci. Rep., 5, 9793 (2015).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to express their sincerest gratitude to Dr M. Singh for use of the NanoSight500 (Biochemistry Department UKZN) and Electron Microscopy Unit (UKZN) facilities. This project was supported by the National Research Foundation, South Africa (Grant Number: 100237).

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

Funding

The authors would like to thank the College of Health Science (UKZN) and the National Research Foundation (NRF) for funding this project.

Author information

Authors and Affiliations

  1. Discipline of Human Physiology, School of Laboratory Medicine & Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa

    Wendy Phoswa & Kogi Moodley

  2. Pearson Institute of Higher Education, Faculty of Applied Science, Johannesburg, 2153, South Africa

    Preenan Pillay

  3. Nuffield Department of Women’s and Reproductive Health, Women’s Center, John Radcliffe Hospital, University of Oxford, Oxford, 38655, UK

    Preenan Pillay

  4. Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, 4000, Republic of South Africa

    Ajit Kumar

  5. Nelson R. Mandela School of Medicine, School of Laboratory Medicine & Medical Sciences. College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa

    Irene Mackraj

Authors
  1. Wendy Phoswa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Preenan Pillay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ajit Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kogi Moodley
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Irene Mackraj
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Irene Mackraj.

Ethics declarations

Ethical approval for this study was obtained from the University of KwaZulu Natal Biomedical Research Ethics Committee on 11/07/16. The BREC reference number allocated to this study is BE230/16.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Phoswa, W., Pillay, P., Kumar, A. et al. The Influence of Bovine Serum Albumin Modified Titanium Dioxide Nanoparticles on Myoblast Cytotoxicity. Pharm Chem J 54, 883–890 (2020). https://doi.org/10.1007/s11094-020-02292-x

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11094-020-02292-x

Keywords

Search

Navigation