A method of extracting mangiferin from dried powdered leaves from mango trees using the solvent mixture isopropanol and water (60:40 v/v) was used. The yields of technical product and purified mangiferin recrystallized from a mixture of isopropanol and water (50:50 v/v) were 1.46% (purity 88.6%) and 0.8% (purity 98.2%). The chemical structure of mangiferin was confirmed by UV-VIS and IR spectrophotometry, electrospray mass spectrometry, and 1H and 13C NMR spectroscopy. Technical product and mangiferin were tested for biological activity in terms of blockade of α-glucosidase, as compared with acarbose. Mangiferin provided significantly greater blockade of α-glucosidase than acarbose, with IC50 values of 11.18 and 5.82 μg/ml for technical product and mangiferin respectively and 199.47 μg/ml for acarbose. These results are important preliminary investigations for further investigation of mangiferin and the development of therapeutic formulations of this highly active hypoglycemic substance.
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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 51, No. 9, pp. 44 – 48, September, 2017.
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Vo, T.H.T., Nguyen, T.D., Nguyen, Q.H. et al. Extraction of Mangiferin from the Leaves of the Mango Tree Mangifera indica and Evaluation of its Biological Activity in Terms of Blockade of α-glucosidase. Pharm Chem J 51, 806–810 (2017). https://doi.org/10.1007/s11094-017-1697-x
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DOI: https://doi.org/10.1007/s11094-017-1697-x