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Development of a Method for Determining the Amino Acid Composition of the Pharmaceutical Substance “Glatiramer Acetate” by NMR Spectroscopy

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Pharmaceutical Chemistry Journal Aims and scope

The acid hydrolysis procedure for glatiramer acetate was modified and the characteristic signals in the 1H and 13C spectra were identified, allowing its amino acid composition to be determined. The relative molar contents of amino acids in glatiramer acetate measured by HPLC-MS and by 13C and 1H NMR had close values. 13C NMR was found to be a more precise method for assaying the amino acid composition of glatiramer acetate than 1H NMR.

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Authors and Affiliations

  1. National Center for Expert Assessment of Medicines, “Russian Ministry of Health”, 8 Petrovskii Boulevard, 127051, Moscow, Russia

    N. E. Kuz’mina, S. V. Moiseev, V. I. Krylov, A. A. Kutin, V. A. Yashkir & V. A. Merkulov

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  1. N. E. Kuz’mina
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  2. S. V. Moiseev
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  3. V. I. Krylov
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  4. A. A. Kutin
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  5. V. A. Yashkir
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  6. V. A. Merkulov
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Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 51, No. 3, pp. 45 – 48, March, 2017.

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Kuz’mina, N.E., Moiseev, S.V., Krylov, V.I. et al. Development of a Method for Determining the Amino Acid Composition of the Pharmaceutical Substance “Glatiramer Acetate” by NMR Spectroscopy. Pharm Chem J 51, 226–229 (2017). https://doi.org/10.1007/s11094-017-1587-2

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  • DOI: https://doi.org/10.1007/s11094-017-1587-2

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