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Study on Fundamental Process of Codonopsis pilosula Desulfurization and In Vitro Antibacterial Activity of its Polysaccharide Extract on Escherichia coli

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Pharmaceutical Chemistry Journal Aims and scope

The present study was aimed to optimize the conditions of Codonopsis pilosula desulfurization using orthogonal experiment analysis with four independent variables including desulfurization temperature, time, ultrasonic power, and ratio of ethanol to raw material. Aspects of the antibacterial activity of polysaccharides extracted from sulfur-fumigated C. pilosula and after desulfurization by the agar-well diffusion method against Escherichia coli have also been studied. The results showed that the optimum extraction conditions were as follows: desulfurization temperature, 45°C; desulfurization time, 50 min; ultrasonic power, 700 W; and ethanol to raw material ratio, 10 : 1. Under these conditions, the desulfurization rate was 55.4%. The antibacterial activity of polysaccharides extracted from desulfurized C. pilosula was higher than that of polysaccharides extracted from sulfur-fumigated herbs, with the MIC values of 35 and 70 mg/mL, respectively.

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References

  1. H. Y. Sun, Z. B. Li, W. X. Liu, et al., Asian J. Chem., 26, 298 – 302 (2014).

    CAS  Google Scholar 

  2. X. Jiang, L. F. Huang, S. H. Zheng, and S. L. Chen, Phytomedicine, 20, 97 – 105 (2013).

    Article  PubMed  CAS  Google Scholar 

  3. X. Jin, L. Y. Zhu, H. Shen, et al., Food Chem., 135, 1141 – 1147 (2012).

    Article  PubMed  CAS  Google Scholar 

  4. L. Liu, X. M. Zhu, Q. J. Wang, et al., Phytochemistry, 71, 1514 – 1520 (2010).

    Article  PubMed  CAS  Google Scholar 

  5. P. Y. Hayes, R. Lehmann, K. Penman, et al., Phytochem. Anal., 17, 251 – 254 (2006).

    Article  PubMed  CAS  Google Scholar 

  6. B. Z. Duan, L. F. Huang, and S. L. Chen, Phytomedicine, 19, 562 – 568 (2012).

    Article  PubMed  CAS  Google Scholar 

  7. Y. X. Sun, and J. C. Liu, Int. J. Biol. Macromol., 43, 279 – 282 (2008).

    Article  CAS  Google Scholar 

  8. Y. X. Sun, J. C. Liu, and J. F. Kennedy, Carbohydr. Polym., 80, 949 – 953 (2010).

    Article  CAS  Google Scholar 

  9. M. P. Wong, T. C. Chiang, and H. M. Chang, Planta Med., 49, 60 (1983).

    Article  PubMed  CAS  Google Scholar 

  10. Z. T. Wang, T. B. Ng, H. W. Yeung, and G. J. Xu, Gen. Pharmacol., 27, 1347 – 1354 (1996).

    Article  PubMed  CAS  Google Scholar 

  11. H. Nörr and H. Wagner, Planta Med., 60, 494 – 495. (1994).

    Article  PubMed  Google Scholar 

  12. H. Luo, S. Lin, F. Ren, et al., J. Food Protect., 70, 1440 – 1445 (2007).

    Google Scholar 

  13. B. E. Shan, Y. Yoshida, T. Sugiura, and U. Yamashita, Int. J. Immunopharmacol., 21, 149 – 154 (1999).

    Article  PubMed  CAS  Google Scholar 

  14. X. Zhang, C. Zhu, L. Hu, et al., Tradit. Chin. Drug Res. Clin. Pharmacol., 14, 174 – 176 (2003).

    Google Scholar 

  15. G. Yang, F. Li, H. Liu, and F. Xian, Pharmacol. Clin. Chin. Mater. Med., 21, 39 – 40 (2005).

    Google Scholar 

  16. J. Chen, H. Jin, L. Sun, et al., Patent No. CN102749410-A (2012).

  17. W. X. Chen, J. Y. Chen, H. M. Wu, et al., Int. J. Biol Macromol., 69, 244 – 251 (2014)

    Article  PubMed  CAS  Google Scholar 

  18. J. C. Liu, S. Miao, X. C. Wen, and Y. X. Sun, Carbohydr. Polym., 78, 704 – 709 (2009).

    Article  CAS  Google Scholar 

  19. M. Dubois, K. A. Gilles, J. K. Hamilton, et al., Anal Chem., 28, 350 (1956).

    Article  CAS  Google Scholar 

  20. C. Z. Wang, H. H. Aung, M. Ni, et al., Planta Med., 73, 669 – 674. (2007).

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  21. P. Y. Hayes, R. Lehmann, K. Penman, et al., Analysis, 17, 251 – 254 (2006).

    CAS  Google Scholar 

  22. F. J. Gui, X. W. Yang, L. Y. Li, and J. M. Tian, J. Chromatogr. B, 850, 1 – 6 (2007).

  23. Z. Hromadkova and A. Ebringerova, Ultrason. Sonochem., 10, 127 – 133 (2003).

    Article  PubMed  CAS  Google Scholar 

  24. Y. Wang, Z. Cheng, J. Mao, et al., Carbohydr. Polym., 77, 713 – 717 (2009).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was financially supported by the Shanxi province science and technology key project of China (20130313015-2). It was also supported by the National Natural Science Foundation of China (31401319), the Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi of China (20151006), and Shanxi Province Science Foundation for Youths (2015021146). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Author Contributions

Conceived and designed the experiments: WYZ. Performed the experiments, contributed reagents/materials/analysis tools, and wrote the paper: WYZ, ERW.

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Authors and Affiliations

  1. College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, Taiyuan, 030021, P. R. China

    Zhao Yuying & Wang Erbing

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  1. Zhao Yuying
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  2. Wang Erbing
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Yuying, Z., Erbing, W. Study on Fundamental Process of Codonopsis pilosula Desulfurization and In Vitro Antibacterial Activity of its Polysaccharide Extract on Escherichia coli . Pharm Chem J 49, 782–787 (2016). https://doi.org/10.1007/s11094-016-1371-8

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  • DOI: https://doi.org/10.1007/s11094-016-1371-8

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