The chromatographic analysis of hydrochlorothiazide (HCT) has been carried out by simple high-performance liquid chromatography (HPLC) procedure, which has been developed for determining HCT in pharmaceutical tablets using different mobile phases in certain ratios. The chromatographic studies of variation of the HPLC detector signal depending on the mobile phase, flow rate, detection wavelength, and column temperature have been analyzed fin order to find the optimal experimental conditions for HCT determination. The developed HPLC technique has been successfully applied to quantitative analysis of HCT in its commercial tablets with a mean recovery of 94.96 ± 1.004%.
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References
B. Beermann, M. Groschinsky-Grind and A. Rosén, Clin. Pharmacol. Ther., 19, 531–537 (1976).
J. D. Duarte and R. M. Cooper-DeHoff, Expert Rev. Cardiovasc. Ther., 8, 793 – 802 (2010).
Hydrochlorothiazide, American Society of Health-System Pharmacists (2011).
N. Erk, Pharmazie, 58, 543 – 548 (2003).
R. Gangola, S. Kaushik, and P. Sharma, J. Appl. Pharm. Sci., 1, 46 – 49 (2011).
B. Burcin, G. Mehmet, T. B. Dogan, et al., J. AOAC Intern., 96, 42 – 51 (2013).
D. Ivanovic, M. Medenica, B. Jancic, et al., Acta Chromatogr., 18, 143 – 156 (2007).
S. S. Qutab, S. N. Razzaq, M. Ashfaq, et al., Acta Chromatogr., 19, 119 – 129 (2007).
I. Violeta, Z. Dragica, and S. Marina, Macedonian Pharm. Bull. , 51, 23 – 28 (2005).
G. N. Menon and L. B. White, J. Pharm. Sci., 70, 1083 – 1085 (1981).
D. Farthinq, I. Fakhry, E. B. Ripley, et al., J. Pharm. Biomed. Anal., 17, 1455 – 1459 (1998).
M. J. Cooper, A. R. Sinaiko, M. W. Anders, et al., Anal. Chem., 48, 1110 – 1111 (1976).
S. Bhagwate and N. J. Gaikwad, J. Appl. Pharm. Sci., 3, 88 – 92 (2013).
I. F. Al-Momani, Turk. J. Chem., 25, 49 – 54 (2001).
S. Sahoo, P. K. Panda, S. K. Mishra, et al., Asian J. Pharm. Clin. Res., 5, 136 – 138 (2012).
S. B.Wankhede, M. R. Tajne, K. R. Gupta, et al., Ind. J. Pharm. Sci., 69, 298 – 300 (2007).
M. B. Gholirand and M. Khodadadian, Electroanalysis, 25, 1263 – 1270 (2013).
O. Abdel Razak, J. Pharm. Biomed. Anal., 34, 433 – 440 (2004).
ACKNOWLEDGEMENTS
The author would like to thank Mr. Awad Alqarni and Ahmad Alribdy at Qassim Pharmaceutical Plant Buraydah (Spimaco Addwaeih, Saudi Arabia) for their assistances and supplying standard drug.
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Alghamdi, A.F. Quantitative Analysis of Hydrochlorothiazide and Its Determination in a Pharmaceutical Preparation by HPLC. Pharm Chem J 48, 843–847 (2015). https://doi.org/10.1007/s11094-015-1207-y
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DOI: https://doi.org/10.1007/s11094-015-1207-y