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Determination of lipid-soluble components in lutein forte

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Pharmaceutical Chemistry Journal Aims and scope

An HPLC method for quantitative determination of lutein, zeaxanthin, retinyl acetate, and tocopheryl acetate in Lutein Forte was developed. The resolution of peaks as a function of two- and three-component mobile phases (MP) was studied. It was shown that Lutein Forte contained a third carotinoid in addition to the declared carotinoids lutein and zeaxanthin. An experimental planning method established that the optimum combination for efficient separation of lutein and zeaxanthin (R = 1.52) and analysis time (24 min) was MP composition CH3CH:dioxane:H2O 88:7:5. The contents of lutein, zeaxanthin, retinyl acetate, and tocopheryl acetate corresponded according to the quantitative determination results to those declared. The relative uncertainty of the average results was less than 1.28%.

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Acknowledgments

We thank Prof. I. Ya. Kon’ (Food Institute, RAMS) for supplying WSS lutein and Prof. E. A. Katsman (MSUFCT) for valuable recommendations regarding the formulation of the mathematical model experiment.

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Authors and Affiliations

  1. Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia

    E. I. Finkelshtein, E. A. Belozertseva, E. V. Trifonova & V. I. Shvets

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  1. E. I. Finkelshtein
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  2. E. A. Belozertseva
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  3. E. V. Trifonova
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  4. V. I. Shvets
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Corresponding author

Correspondence to E. I. Finkelshtein.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 47, No. 6, pp. 39 – 43, June, 2013.

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Finkelshtein, E.I., Belozertseva, E.A., Trifonova, E.V. et al. Determination of lipid-soluble components in lutein forte. Pharm Chem J 47, 325–329 (2013). https://doi.org/10.1007/s11094-013-0954-x

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  • DOI: https://doi.org/10.1007/s11094-013-0954-x

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