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Characterization of the composition and antioxidant activity of plant extracts by HPLC with UV and amperometric detection

  • Medicinal Plants
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Pharmaceutical Chemistry Journal Aims and scope

High-performance liquid chromatography with UV and amperometric detection has been used to analyze qualitatively and quantitatively phenolic extracts of several plants used in the production of drugs, food, and cosmetics. Phenolic compounds in aqueous alcohol, alcohol, and propyleneglycol (PPG) extracts were identified as groups using a previously constructed database of the spectra and retention times of 74 phenolic compounds. The antioxidant activity (AA) of the extracts was measured as the sum of peaks on the chromatograms after the amperometric detector. The AA of the alcohol extracts correlated with the flavonoid content. Extracts obtained using supercritical PPG had the maximum AA.

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Authors and Affiliations

  1. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, 119991, Russia

    E. N. Semenistaya & O. G. Larionov

Authors
  1. E. N. Semenistaya
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  2. O. G. Larionov
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Correspondence to E. N. Semenistaya.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 42, No. 9, pp. 43–48, September, 2008.

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Semenistaya, E.N., Larionov, O.G. Characterization of the composition and antioxidant activity of plant extracts by HPLC with UV and amperometric detection. Pharm Chem J 42, 539–544 (2008). https://doi.org/10.1007/s11094-009-0167-5

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  • DOI: https://doi.org/10.1007/s11094-009-0167-5

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