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Determining amlodipine in blood plasma by liquid chromatography coupled to tandem mass spectrometry

  • Structure of Chemical Compounds, Methods of Analysis and Process Control
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Pharmaceutical Chemistry Journal Aims and scope

Asimple and rapid high-performance liquid chromatography (HPLC) method with tandem mass spectrometry (TMS) detectoin has been developed for the determination of amlodipine in blood plasma. The HPLC system is coupled to a TMS detector via an electrospray ionization (ESI) interface in the positive-ion mode. The plasma samples were extracted either with a diethylether:hexane (8:2, v/v) mixture of by means of solid-phase extraction with Oasis HLB 1-cc cartridges (Waters, USA). The analytes were chromatographed under reversed-phase conditions on a Zorbax 300 SB-C18 column. The proposed HPLC/TMS procedure was successfully used for the study of the clinical pharmacokinetics of amlodipine and other calcium-channel blockers.

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References

  1. H. R., Clin. Cornerstone, 6, No. 4, 53–66 (2004).

  2. J. H. Lin and A. Y. H. Lu, Pharm. Rev., 49(4), 404–449 (1997).

    Google Scholar 

  3. V. G. Kukes, Clinical Pharmacology [in Russian], GEOTAR Meditsina, Moscow (1999).

    Google Scholar 

  4. M. V. Leonova, Kach. Klin. Praktika, No. 3, 23–30 (2005).

  5. A. Zarghi, S. M. Foroutan, A. Shafaati, and A. Khoddam, Farmaco, 60(9), 789–792 (2005).

    Article  PubMed  CAS  Google Scholar 

  6. G. Bahrami and S. Mirzaeei, J. Pharm. Biomed. Anal., 36(1), 163–168 (2004).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Mental Health Research Center, Russian Academy of Medical Sciences, Moscow, Russia

    I. I. Miroshnichenko, A. A. Lelishentsev & Yu. M. Kalmykov

  2. Chemical Diversity Research Institute, Khimki, Moscow Region, Russia

    Yu. A. Fedotov & A. A. Ivashchenko

Authors
  1. I. I. Miroshnichenko
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  2. A. A. Lelishentsev
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  3. Yu. M. Kalmykov
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  4. Yu. A. Fedotov
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  5. A. A. Ivashchenko
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Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 42, No. 8, pp. 54–56, August, 2008.

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Miroshnichenko, I.I., Lelishentsev, A.A., Kalmykov, Y.M. et al. Determining amlodipine in blood plasma by liquid chromatography coupled to tandem mass spectrometry. Pharm Chem J 42, 493–495 (2008). https://doi.org/10.1007/s11094-008-0158-y

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  • DOI: https://doi.org/10.1007/s11094-008-0158-y

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