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Immobilization of Penicillium solitum lipase on the carbon fiber material “Dnepr-MN”

  • Drug Synthesis Methods and Manufacturing Technology
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Abstract

A method for immobilizing Penicillium solitum lipase (the medicinal agent Solizyme) on the activated carbon fiber material “Dnepr-MN” is proposed; using a matrix with total pore volumes of 0.41–1.3 cm3/g, the method allows the enzyme:matrix ratio (1:30–1:60) to be controlled, yielding preparations with a specified lipolytic activity (20–40 LU/mg of agent) at quantitative yield (91–97%). Studies of the pH and temperature stability, functioning in acid media, and desorption demonstrated stabilization and prolongation of the lipolytic activity of the immobilized lipase.

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References

  1. T. I. Davidenko, Ukr. Khim. Zh., 65(9), 48–55 (1999).

    CAS  Google Scholar 

  2. T. I. Davidenko, Visnik. ONU, 8(4), 135–147 (2003).

    Google Scholar 

  3. N. Yu. Pimonenko, V. P. Sergeev, Yu. A. Sokolova, et al., in: Proceedings of the IV Republican Conference “Medical Sorbents and their Mechanisms of Therapeutic Actions” [in Russian], Donetsk (1988), pp. 23–24.

  4. E. V. Sevast'yanova and T. I. Davidenko, Prikl. Biokhim. Mikrobiol., 29(3), 375–380 (1993).

    Google Scholar 

  5. E. V. Sevast'yanova, T. I. Davidenko, V. P. Sergeev, et al., Khim.-Farm. Zh., 26(6), 40–42 (1992).

    Google Scholar 

  6. V. G. Nikolaev and V. V. Streklo, Hemosorption on Activated Charcoals [in Russian], Naukova Dumka, Kiev (1979).

    Google Scholar 

  7. I. M. Gracheva, Yu. P. Grachev, M. S. Mosichev, et al., Laboratory Handbook in the Technology of Enzyme Preparations. Textbook for Doctors [in Russian], Legkaya i Pishchevaya Promyshlennost', Moscow (1982).

    Google Scholar 

  8. E. E. Hartree, Anal. Biochem., 48(7), 422–425 (1972).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. A. V. Bogatskii Physico-Chemical Institute, Ukrainian National Academy of Sciences, Odessa, Ukraine

    I. I. Romanovskaya, G. I. Bondarenko & T. I. Davidenko

Authors
  1. I. I. Romanovskaya
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  2. G. I. Bondarenko
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  3. T. I. Davidenko
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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 42, No. 6, pp. 49–51, June, 2008.

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Romanovskaya, I.I., Bondarenko, G.I. & Davidenko, T.I. Immobilization of Penicillium solitum lipase on the carbon fiber material “Dnepr-MN”. Pharm Chem J 42, 360–362 (2008). https://doi.org/10.1007/s11094-008-0127-5

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  • DOI: https://doi.org/10.1007/s11094-008-0127-5

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