Abstract
The antioxidant activity of aqueous and ethanol extracts of iris (Iris germanica L., family Iridaceae) has been evaluated in vitro using various antioxidant assays, including reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activities. Both aqueous and ethanol extracts exhibit strong total antioxidant activity, showing 95.9, 88.4, 79.9% and 90.5, 78.0, 65.3% inhibition on peroxidation of linoleic acid emulsion in concentrations of 10, 30, and 50 µg-ml, respectively. Both extracts also possess effective reducing power and exhibit free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activities in concentrations of 20, 40, and 60 µg-ml. The antioxidant properties were compared to those of reference antioxidants (BHA, BHT, and α-tocopherol). In addition, the total content of phenolic compounds in both aqueous and ethanol iris extracts has been determined as gallic acid equivalent. The results indicate that iris has in vitro antioxidant properties, which can be the major factor responsible for the inhibition of lipid peroxidation.
Similar content being viewed by others
References
S. I. Madhavi, S. S. Deshpande, and S. S. Salunkhe, Food Antioxidants: Technological, Toxicological, Health Perspective, Marcel Dekker, New York (1996), p. 1.
R. G. Culter, Antioxidants, Aging, and Longevity, in: Free Radicals in Biology, W. A. Pryor (ed.), Academic Press, Orlando, FL (1984), Vol. 6, p. 371.
D. Harman, The Free-Radical Theory of Aging, in: Free radicals in biology, W. A. Pryor (ed.), Academic Press, Orlando, FL (1982), Vol. 5.
K. Yagi, Chem. Phys. Lipids, 45, 337 (1987).
T. Mizutani, H. Nomura, K. Nakanishi, and S. Fujita, Toxicol. Appl. Pharmacol., 87, 166–176 (1987).
G. M. Williams, Y. Maeura, J. H. Weisburger, Cancer Lett., 19, 55–60 (1983).
Y. Noda, T. Kaneyuki, A. Mori, and L. Packer, J. Agric. Food Chem., 50, 166–171 (2002).
S. Schaffer, S. Schmitt-Schillig, W. E. Muller, and G. P. Eckert, J. Physiol. Pharmacol., 56, 115–124 (2005).
T. Tsuda, K. Ohshima, S. Kawakishi, and T. Osawa, J. Agric. Food Chem., 42, 248–251 (1994).
A. Baytop and T. Baytop, Flora of Turkey, University Press, Edinburgh (1984), Vol. 8, p. 392.
Y. Muto, H. Ichikawa, O. Kitagawa, et al., Yakugaku Zasshi, 114, 980–994 (1994).
Y. Miyake, H. Ito, and T. Yoshida, Can. J. Chem., 75, 734–741 (1997).
I. Orhan, B. Sener, T. Hashimoto, et al., Fitoterapia, 73(4), 316–319 (2002).
C. Raymond, Trans. Soc. Pharmacol. Montpellier, 31, 325–334 (1971).
H. Mitsuda, K. Yuasumoto, and K. Iwami, Eiyo to Shokuryo, 19, 210–214 (1996).
P. D. Duh,, Y. Y. Tu, and G. C. Yen, Lebnesmitt. Wiss. Technol., 32, 269–277 (1999).
M. Oyaizu, Jpn. J. Nutr., 44, 307–315 (1986).
F. Liu, V. E. C. Ooi, and S. T. Chang, Life Sci., 60, 763–771 (1997).
X. Y. Ye, H. X. Wang, F. Liu, and T. B. Ng, Int. J. Biochem. Cell Biol., 32, 235–241 (2000).
M. S. Blois, Nature, 26, 1199–1200 (1958).
T. C. P. Dinis, V. M. C. Madeira, and L. M. Almeida, Arch. Biocem. Biophys., 315, 161–169 (1994).
R. J. Ruch, S. J. Cheng, and J. E. Klaunig, Carcinogenesis, 10, 1003–1008 (1989).
K. Slinkard and V. L. Singleton, Am. J. Enol. Viticult., 28, 49–55 (1977).
S. Meir, J. Kanner, B. Akiri, and S. P. Hadas, J. Agric. Food Chem., 43, 1813–1815 (1995).
A. T. Diplock, Free Radic. Res., 27, 511–532 (1997).
A. Yildirim, A. Mavi, and A. A. Kara, J. Agric. Food Chem., 49, 4083–4089 (2001).
J. R. Soares, T. C. P. Dins, A. P. Cunha, and L. M. Ameida, Free Radic. Res., 26, 469–478 (1997).
F. Yamaguchi, T. Ariga, Y. Yoshimira, and H. Nakazawa, J. Agric. Food Chem., 48, 180–185 (2000).
M. H. Gordon, The Mechanism of the Antioxidant Action in vitro, in: Food Antioxidants, B. J. F. Hudson (ed.), Elsevier, London (1990), pp. 1–18.
T. Hatano, R. Edamatsu, A. Mori, Y. Fujita, and E. Yasuhara, Chem. Pharm. Bull., 37, 2016–2021 (1989).
Y. S. Velioglu, G. Mazza, L. Gao, and B. D. Oomah, J. Agric. Food Chem., 46, 4113–4117 (1998).
M. Tanaka, C. W. Kuei, Y. Nagashima, and T. Taguchi, Nippon Suisan Gakkaishi, 54, 1409–1414 (1998).
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Khimiko-Farmatsevticheskii Zhurnal, Vol. 41, No. 8, pp. 13–18, August, 2007.
Rights and permissions
About this article
Cite this article
Nadaroğlu, H., Demir, Y. & Demir, N. Antioxidant and radical scavenging properties of Iris germanica . Pharm Chem J 41, 409–415 (2007). https://doi.org/10.1007/s11094-007-0089-z
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11094-007-0089-z