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Doxorubicin complexes with copper ions of the active center of ceruloplasmin

  • Structure of Chemical Compounds, Methods of Analysis and Process Control
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Abstract

Features of the interaction of doxorubicin (DR) with Cu2+ ions of the active center of ceruloplasmin (CP) have been studied by electronic spectroscopy. It is established that DR · Cu2+ CP and DR complexes with inorganic copper salts (Cu(CH3COO)2 and CuSO4) have the same chromophore. An equation establishing a relationship between the electronic absorption at 540 nm (chromophore absorption region) and the concentration of DR involved in the complex formation is proposed. Using this dependence in accordance with the Benesi-Hildebrand theory, the complex formation constant was approximately evaluated as Kp = 4.5 × 106. Effective complex formation between DR and Cu2+ ions of the CP active center creates prerequisites for the removal of Cu2+ ions from CP and, consequently, for a decrease in CP oxidase activity.

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Authors and Affiliations

  1. Nizhni Novgorod State Medical Academy, Nizhni Novgorod, Russia

    N. B. Mel’nikova, A. A. Volkov & S. V. Kononova

  2. ImBio Company, MICROGEN Research and Production Corporation, Nizhni Novgorod, Russia

    T. A. Krainova, L. M. Efremova & V. V. Anastasiev

Authors
  1. N. B. Mel’nikova
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  2. A. A. Volkov
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  3. S. V. Kononova
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  4. T. A. Krainova
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  5. L. M. Efremova
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  6. V. V. Anastasiev
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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 40, No. 5, pp. 49–53, May, 2006.

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Mel’nikova, N.B., Volkov, A.A., Kononova, S.V. et al. Doxorubicin complexes with copper ions of the active center of ceruloplasmin. Pharm Chem J 40, 284–288 (2006). https://doi.org/10.1007/s11094-006-0110-y

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  • DOI: https://doi.org/10.1007/s11094-006-0110-y

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