Cytotoxicity and apoptotic effects of polymer coated copper oxide nanoparticles synthesized via SLM in mesenchymal stem cells
- 138 Downloads
Copper oxide inclusions were incorporated in polymer matrix (polycarbonate/PC/, polyetheretherketone/PEEK/etc.) during selective laser melting process. The aim of this study was to observe the cytotoxicity and apoptotic effects of the copper oxide nanoparticles on a culture of multipotent mesenchymal stem cells after 3D laser bioprinting. The matrices of polymer coated Cu/CuO nanoparticles (<100 nm) were investigated by X-ray diffraction analysis and scanning electron microscopy techniques. The viable cells were counted using light microscopy. It was determined that Cu/CuO nanoparticles have a strong toxic effect on PC matrix unlike background high biocompatible PEEK matrix, which smoothes apoptotic effect. We also discuss the implications of our findings regarding the effects of the intrinsic toxic properties of Cu/CuO nanoparticles, and concluded that the apparent toxicities of metal oxide NPs can largely be understood as a matter of particle toxicity.
Keywords3D laser bioprinting Copper nano particles (NP) Biopolymers Multipotent mesenchymal stem cells (MMSC) Cytotoxicity
The study was supported by the grant of the Russian Science Foundation (Project No. 15-19-00208).
- Murray, P.E., García Godoy, C., García Godoy F.: How is the biocompatibility of dental biomaterials evaluated? Med. Oral Patol. Oral Cir. Bucal 12(3), E258–E266 (2007)Google Scholar
- Shafagh, M., Rahmani, F., Delirezh, N.: CuO nanoparticles induce cytotoxicity and apoptosis in human K562 cancer cell line via mitochondrial pathway, through reactive oxygen species and P53. Iran. J. Basic Med. Sci. 18(10), 993–1000 (2015)Google Scholar
- Shishkovsky, I.: Laser synthesis of functional mesostructures and 3D parts. Fizmatlit Publ, Moscow (2009)Google Scholar
- Shishkovskii, I.V., Morozov, YuG, Fokeev, S.V., et al.: Laser synthesis and comparative testing of a three-dimensional porous matrix of titanium and titanium nickelide as a repository for stem cells. Powder Metall. Met. Ceram. 50(9/10), 606–618 (2012). doi: 10.1007/s11106-012-9366-9 CrossRefGoogle Scholar
- Shishkovsky, I.V.: SLS design of porous drug delivery system from porous nitinol. Nano- i mikrosistemnaya tekhnika 9, 39–43 (2012)Google Scholar
- Volchkov, S.E., Tumina, O.V., Topovski, A.N., et al.: Comparative characteristics of MMSC from different sources. Cell Transplantol. Tissue Eng. 3, 8–9 (2009)Google Scholar