Skip to main content
SpringerLink
Log in

The Development of Leukocyte Counter Using Fluorescence Imaging Analysis

  • Published:
Optical and Quantum Electronics Aims and scope Submit manuscript

Abstract

Image cytometry centrifugation (ICC) method has been developed and applied to count residual leukocytes in transfusion blood products for quality assurance testing. The standard leukocyte fluorescence image profile was established by averaging the actual fluorescence images of leukocytes. It was used for development of an optimum image analysis algorithm to avoid misinterpretation of the dust contaminated from the environment. The centrifugal concentration process of the ICC method was investigated by a computer simulation, and it was found that it caused leukocyte overlapping and limited the enumeration range. By introducing a double thresholding algorithm to the fluorescence images analysis and correcting the number of the overlapped leukocytes, we succeeded in improving the accuracy and the measurement range up to 25 [WBC/μl] of the ICC method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • AABB, Blood Transfusion Therapy: A Physician’s Handbook, 7th edn., p. 80, 2002.

  • AABB Technical Manual, 12th edn., Bethsda, AABB, p. 722, 1996.

  • M.R. Adams D.K. Johnson M.P. Busch (1997) Transfusion 37 29 Occurrence Handle10.1046/j.1537-2995.1997.37197176948.x Occurrence Handle9024487

    Article  PubMed  Google Scholar 

  • Blood Products Advisory Committee, Summary for Topics II, Gaithersburg, MD, December 13–14, 2001, http://www.fda.gov/ohrms/dockets/ac/01/briefing/3817b1_06_williams1.pdf

  • S. Dizik (1997) Transfus. Med. Rev 11 44 Occurrence Handle9031490

    PubMed  Google Scholar 

  • Nagahashi H.K. Yabusaki H. Matsui T. Hirono N. Yokokawa M. Satake K. Tadokoro T. Juji (2000) Vox Sang 79 34 Occurrence Handle10.1159/000031203 Occurrence Handle10971212

    Article  PubMed  Google Scholar 

  • J. Neumuller D.W.M. Schwartz W.R. Mayr (1997) Vox Sang 73 220 Occurrence Handle10.1046/j.1423-0410.1997.7340220.x Occurrence Handle9407639

    Article  PubMed  Google Scholar 

  • Yabusaki, K., T. Hirono and H. Matsui. XIIth International Symposium on Technological Innovations in Laboratory Hematology, Workshop on New Technology in Laboratory Hematology, Kobe, Japan, September 9–11, 79, 1999.

  • K. Yabusaki N. Saitoh T. Hirono H. Matshui H. Okada (2000) Jpn. J. Appl. Phys 39 3641 Occurrence Handle10.1143/JJAP.39.3641

    Article  Google Scholar 

  • J.V. Watson (2001) Cytometry (Communications in Clinical Cytometry) 46 1 Occurrence Handle10.1002/1097-0320(20010215)46:1<1::AID-CYTO1032>3.0.CO;2-3

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Laboratories, Electronics and Optics Res. Labs., Kowa Company, Ltd, 3-3-1, Chofugaoka, Chofu, Tokyo, 182-0021, Japan

    Taisuke Hirono

  2. Kowa Research Institute, Kowa Company, Ltd, Japan

    Katsumi Yabusaki

  3. Dept. of Mech. Eng. and Intel. Sys, University of Electro-Communications, Japan

    Taisuke Hirono & Yukio Yamada

Authors
  1. Taisuke Hirono
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Katsumi Yabusaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yukio Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Taisuke Hirono.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hirono, T., Yabusaki, K. & Yamada, Y. The Development of Leukocyte Counter Using Fluorescence Imaging Analysis. Opt Quant Electron 37, 735–753 (2005). https://doi.org/10.1007/s11082-005-8341-9

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11082-005-8341-9

Keywords

Search

Navigation