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Dorsal Skin Fold Chamber for High Resolution Multiphoton Imaging

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Abstract

The advantages offered by multiphoton microscopy enabled this technique to be applied for in vivo understanding of physiological processes. However, multiphoton intravital microscopy also requires associated technologies to be developed. In this work, we detailed the design of a dorsal skin fold chamber made of titanium alloy that allow high resolution multiphoton fluorescence and second harmonic generation (SHG) microscopy to be achieved. We demonstrate that our apparatus is capable of obtaining high resolution, images of blood vessels and collagen matrix in nude mice. Such an imaging chamber will allow physiological processes to be investigated at high resolution

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Authors and Affiliations

  1. Department of Physics, National Taiwan University, Taipei, 106, Taiwan, ROC

    Feng-Chieh Li, Chun-Chin Wang, Wen Lo & Chen-Yuan Dong

  2. Department of Dermatology, National Taiwan University Hospital, Taipei, 100, Taiwan, ROC

    Sung-Jan Lin & Shiou-Hwa Jee

  3. Department of Dermatology, College of Medicine, National Taiwan University Hospital, Taipei, 100, Taiwan, ROC

    Shiou-Hwa Jee

Authors
  1. Feng-Chieh Li
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  2. Chun-Chin Wang
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  3. Sung-Jan Lin
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  4. Shiou-Hwa Jee
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  5. Wen Lo
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  6. Chen-Yuan Dong
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Corresponding author

Correspondence to Chen-Yuan Dong.

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Li, FC., Wang, CC., Lin, SJ. et al. Dorsal Skin Fold Chamber for High Resolution Multiphoton Imaging. Opt Quant Electron 37, 1439–1445 (2005). https://doi.org/10.1007/s11082-005-4223-4

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  • DOI: https://doi.org/10.1007/s11082-005-4223-4

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