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Peroxidase-ROS interactions

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Abstract

Reactive oxygen species (ROS), such as hydrogen peroxide and superoxide anion radical, have long been recognized as harmful by-products of oxidative metabolism. Under normal physiologic conditions, hydrogen peroxide and superoxide are detoxified by antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx). Heme peroxidases (eosinophil peroxidase (EPO), lactoperoxidase (LPO), myeloperoxidase (MPO), etc.) also consume ROS, but unlike scavenging enzymes, are sources of these species as well. In the present paper, we study a well-tested model of the peroxidase–oxidase (PO) reaction based on horseradish peroxidase (HRP) chemistry with regard to the production and consumption of hydrogen peroxide and superoxide. Our principal results are these:

  1. 1.

    PO reactions can transduce continuing infusions of hydrogen peroxide and superoxide into bounded dynamics.

  2. 2.

    Absent exogenous ROS input, and under conditions that retard hydrogen donor autoxidation, PO reactions can manifest low frequency bursting whereby pulses of ROS are produced at clinically significant intervals.

The relevance of these results to the functional significance of fluctuating ROS concentrations in vivo, to neurodevelopmental and neurodegenerative disease and to episodic and progressive symptomatology is discussed.

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Abbreviations

α-KGDH:

α-ketoglutarate dehydrogenase

ALS:

Amyotrophic lateral sclerosis

AD:

Alzheimer’s disease

ASD:

Autistic spectrum disorder

CAT:

Catalase

CSTR:

Continuous stirred tank reactor

DCP:

Dichlorophenol

EPO:

Eosinophil peroxidase

ETC:

Electron transport chain

GPx:

Glutathione peroxidase

HA:

Hyperammonemia

HRP:

Horseradish peroxidase

LPO:

Lactoperoxidase

MB:

Methylene blue

MPO:

Myeloperoxidase

MS:

Multiple sclerosis

NADH:

Reduced nicotinamide adenine dinucleotide

NADPH:

Reduced Nicotinamide adenine dinucleotide phosphate

PD:

Parkinson’s disease

PO:

Reaction peroxidase oxidase reaction

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TCA:

Cycle (tricarboxylic acid cycle)

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  1. Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, AZ, 85721, USA

    W. M. Schaffer & T. V. Bronnikova

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  1. W. M. Schaffer
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Correspondence to W. M. Schaffer.

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Schaffer, W.M., Bronnikova, T.V. Peroxidase-ROS interactions. Nonlinear Dyn 68, 413–430 (2012). https://doi.org/10.1007/s11071-011-0314-x

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