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The α6 integrin subunit in the developing mouse olfactory bulb

  • Published:
Journal of Neurocytology

Abstract

Integrins are heterodimeric cell surface receptors that mediate developmental events by binding extracellular matrix ligands. Several lines of evidence suggest a role for integrins, specifically the α 6 subunit, in neuronal migration, neurite outgrowth, and axon guidance during olfactory development. Therefore, we undertook an analysis of the expression of the α 6 subunit in the olfactory system of the embryonic and early postnatal mouse to understand the role it may play during neural development. In addition, as a functional assay we examined the developmental effects of the loss of this subunit on olfactory development by analyzing an α 6 knockout (α 6−/−). Immunohistochemical analyses and confocal microscopy were used to examine α 6 expression in the CD-1 embryonic and early postnatal olfactory system and also to examine the organization of the olfactory system in the α 6−/− mouse. In CD-1 mice from E13 to E17, α 6 localizes in radial patterns extending from the core of the olfactory bulb to the nerve layer and colocalizes with RC2, an antibody specific for radial glia. By the day of birth (P0; ∼E19), expression is limited to the external plexiform layer and the olfactory nerve layer, where it colocalizes with laminin and p75. In the α 6−/− mouse, areas of ectopic granule cells were observed in the mitral cell layer of the olfactory bulb. These ectopias coincided with areas of disorganization of the radial glial processes and breaks in the mitral cell layer. These observations suggest a role for α 6 integrin in neural migration during olfactory development, likely secondary to organization of the radial glial scaffold.

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Authors and Affiliations

  1. Department of Neurosurgery, Yale University School of Medicine, New Haven, CT

    Matthew Whitley, Helen Treloar & Charles A. Greer

  2. Department of Neurobiology, Yale University School of Medicine, New Haven, CT

    Charles A. Greer

  3. Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS/INSERM/ULP, Strasbourg, France

    Adele De Arcangelis & Elisabeth Georges Labouesse

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  1. Matthew Whitley
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  2. Helen Treloar
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  3. Adele De Arcangelis
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  4. Elisabeth Georges Labouesse
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  5. Charles A. Greer
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Correspondence to Charles A. Greer.

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Whitley, M., Treloar, H., De Arcangelis, A. et al. The α6 integrin subunit in the developing mouse olfactory bulb. J Neurocytol 34, 81–96 (2005). https://doi.org/10.1007/s11068-005-5049-5

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  • DOI: https://doi.org/10.1007/s11068-005-5049-5

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