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Byrsonima sericea Ethanol Extract Protected PC12 Cells from the Oxidative Stress and Apoptosis Induced by 6-Hydroxydopamine

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Abstract

Parkinson’s disease is characterized by the progressive loss of dopaminergic neurons in the nigrostriatal pathway and oxidative stress is one of the main mechanisms that lead to neuronal death in this disease. Previous studies have shown antioxidant activity from the leaves of Byrsonima sericea, a plant of the Malpighiaceae family. This study aimed to evaluate the cytoprotective activity of the B. sericea ethanolic extract (BSEE) against the cytotoxicity induced by 6-hydroxydopamine (6-OHDA) in PC12 cells, an in vitro model of parkinsonism. The identification of phenolic compounds in the extract by HPLC-DAD revealed the presence of geraniin, rutin, isoquercetin, kaempferol 3-O-β-rutinoside, and quercetin. The BSEE (75–300 µg/mL) protected PC12 cells from toxicity induced by 6-OHDA (25 µg/mL), protected cell membrane integrity and showed antioxidant activity. BSEE was able to decrease nitrite levels, glutathione depletion, and protect cells from 6-OHDA-induced apoptosis. Thus, we suggest that the BSEE can be explored as a possible cytoprotective agent for Parkinson’s disease due to its high antioxidant capacity and anti-apoptotic action.

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Supporting data are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors wish to thank the Brazilian National Research and Development Council (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), and the Research Support Foundation of Ceará (FUNCAP) for financial support in the form of grants and fellowship awards.

Funding

This study did not receive specific funding. The general funding was supported by the Brazilian National Research and Development Council (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), and the Research Support Foundation of Ceará (FUNCAP).

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Authors and Affiliations

  1. Department of Clinical Medicine, Faculty of Medicine, Federal University of Ceará, Rua Professor Costa Mendes, 1608, Fortaleza, CE, 60.430-140, Brazil

    Albert Layo Costa de Assis, Jessica Maria Pessoa Gomes, Tyciane de Souza Nascimento, Mayara Sandrielly Soares de Aguiar & Geanne Matos de Andrade

  2. Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Rua Coronel Nunes de Melo, 1127, Fortaleza, CE, 60.430-275, Brazil

    Patricia de Araújo Rodrigues, Alfaete Vieira Oliveira & Geanne Matos de Andrade

  3. Neuroscience and Behavior Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Rua Coronel Nunes de Melo, 1000, Fortaleza, CE, 60.430-275, Brazil

    Albert Layo Costa de Assis, Patricia de Araújo Rodrigues, Jessica Maria Pessoa Gomes, Tyciane de Souza Nascimento, Alfaete Vieira Oliveira, Mayara Sandrielly Soares de Aguiar & Geanne Matos de Andrade

  4. Natural Product Chemistry Laboratory, State University of Ceará - NUPESA, Avenida Dr. Silas Munguba, 1700 - Itaperi, Fortaleza, CE, 60714-903, Brazil

    Selene Maia de Morais & Ana Livya Moreira Rodrigues

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Contributions

Assis ALC, Gomes JMP, Nascimento TC and Oliveira AV: Cells treatment, biochemical analysis, statistical analysis, results interpretation, discussion, and manuscript preparation. Rodrigues PA, Morais SM and Rodrigues ALM: Plant collection, preparation of the extract and HPLC analysis. Andrade GM, Aguiar MSS, Morais SM: Experimental design, statistical analysis, results interpretation, discussion, and manuscript preparation.

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Correspondence to Mayara Sandrielly Soares de Aguiar or Geanne Matos de Andrade.

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de Assis, A.L.C., de Araújo Rodrigues, P., de Morais, S.M. et al. Byrsonima sericea Ethanol Extract Protected PC12 Cells from the Oxidative Stress and Apoptosis Induced by 6-Hydroxydopamine. Neurochem Res 49, 234–244 (2024). https://doi.org/10.1007/s11064-023-04028-1

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