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Glutamine Maintains Satellite Glial Cells Growth and Survival in Culture

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Abstract

Satellite glial cells (SGCs) tightly surround neurons and modulate sensory transmission in dorsal root ganglion (DRG). At present, the biological property of primary SGCs in culture deserves further investigation. To reveal the key factor for SGCs growth and survival, we examined the effects of different culture supplementations containing Dulbecco’s Modified Eagle Medium (DMEM)/F12, DMEM high glucose (HG) or Neurobasal-A (NB). CCK-8 proliferation assay showed an increased proliferation of SGCs in DMEM/F12 and DMEM/HG, but not in NB medium. Bax, AnnexinV, and propidium iodide (PI) staining results showed that NB medium caused cell death and apoptosis. We showed that glutamine was over 2.5 mM in DMEM/F12 and DMEM/HG, whereas it was absence in NB medium. Interestingly, exogenous glutamine application significantly reversed the poor proliferation and cell death of SGCs in NB medium. These findings demonstrated that DMEM/F12 medium was optimal to get high-purity SGCs. Glutamine was the key molecule to maintain SGCs growth and survival in culture. Here, we provided a novel approach to get high-purity SGCs by changing the key component of culture medium. Our study shed a new light on understanding the biological property and modulation of glial cells of primary sensory ganglia.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request. The authors declared that all the data had been included in the manuscript, and the raw data could be obtained from the corresponding author with reasonable request.

Abbreviations

Bax:

Bcl-2 Associated protein X

DAPI:

4',6-Diamidino-2-phenylindole dihydrochloride

DMEM:

Dulbecco’s Modified Eagle Medium

HG:

High glucose

DRG:

Dorsal root ganglion

GS:

Glutamine synthetase

GFAP:

Glial fibrillary acidic protein

NB:

Neurobasal-A

PI:

Propidium iodide

SGC:

Satellite glial cell

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Acknowledgements

This study was funded by grants from NSFC grant 31871067, 81571073, Shaanxi Science Fund 2020JZ-27, 2018JC-014, Experimental Animal grant SYDW-2017-08, FMMU grant 2021HKYX29, and Tangdu Hospital grant 2018QYTS001, 2021SHRC013.

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Author notes

  1. Na Wei, Ya-Ping Liu and Rui-Rui Wang both contributed equally to this study.

Authors and Affiliations

  1. Institute for Biomedical Sciences of Pain, Tangdu Hospital, Fourth Military Medical University, 569 Xinsi Road, Baqiao, Xi’an, Shaanxi, 710038, People’s Republic of China

    Na Wei, Ya-Ping Liu, Rui-Rui Wang, Zhen-Juan Zhong, Xiao-Liang Wang, Yan Yang, Ting He, Si-Jia Zhao & Yao-Qing Yu

  2. Department of Dermatology, Tangdu Hospital, Fourth Military Medical University, 569 Xinsi Road, Baqiao, Xi’an, 710038, People’s Republic of China

    Huan Wang

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  1. Na Wei
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Correspondence to Huan Wang or Yao-Qing Yu.

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The experimental procedures were approved by the Institutional Animal Care and Use Committee of FMMU.

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Wei, N., Liu, YP., Wang, RR. et al. Glutamine Maintains Satellite Glial Cells Growth and Survival in Culture. Neurochem Res 47, 3635–3646 (2022). https://doi.org/10.1007/s11064-022-03614-z

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