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Neural Differentiation of Human-Induced Pluripotent Stem Cells (hiPSc) on Surface-Modified Nanofibrous Scaffolds Coated with Platelet-Rich Plasma

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Abstract

The field of tissue engineering exploits living cells in a variety of ways to restore, maintain, or enhance tissues and organs. Between stem cells, human induced pluripotent stem cells (hiPSCs), are very important due to their wide abilities. Growth factors can support proliferation, differentiation, and migration of hiPSCs. Platelet-rich plasma (PRP) could be used as the source of growth factors for hiPSCs. In the present study, proliferation and neural differentiation of hiPSCs on surface-modified nanofibrous Poly-l-lactic acid (PLLA) coated with platelet-rich plasma was investigated. The results of in vitro analysis showed that on the surface, which was modified nanofibrous scaffolds coated with platelet-rich plasma, significantly enhanced hiPSCs proliferation and neural differentiation were observed. Whereas the MTT ([3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide]) results showed biocompatibility of surface-modified nanofibrous scaffolds coated with platelet-rich plasma and the usage of these modified nanoscaffolds in neural tissue engineering in vivo is promising for the future.

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Acknowledgements

The authors thank the Nanobiotechnology Research Center of Baqiyatallah University of Medical Sciences for their valuable contributions to this research.

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Authors and Affiliations

  1. Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran

    Reza Moazamiyanfar

  2. Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Raheleh Halabian

  3. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Marzieh Ghollasi

  4. Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands

    Delaram Poormoghadam

  5. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Maliheh Entezari

  6. Immunogenetics Research Center, Department of Medical Biotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

    Seyed Ehsan Endorami

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  1. Reza Moazamiyanfar
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  6. Seyed Ehsan Endorami
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RM, RH, and MG reviewed the literature, outlined, wrote the manuscript. SEE, ME edited the manuscript and DP prepared figures. All authors read and approved the final manuscript.

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Correspondence to Marzieh Ghollasi.

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Moazamiyanfar, R., Halabian, R., Ghollasi, M. et al. Neural Differentiation of Human-Induced Pluripotent Stem Cells (hiPSc) on Surface-Modified Nanofibrous Scaffolds Coated with Platelet-Rich Plasma. Neurochem Res 47, 1991–2001 (2022). https://doi.org/10.1007/s11064-022-03584-2

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