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The Role of Klotho Protein Against Sevoflurane-Induced Neuronal Injury

Neurochemical Research volume 47, pages 315–326 (2022)Cite this article

Abstract

The effects of general anesthetics on the developing brain have aroused much attention in recent years. Sevoflurane, a commonly used inhalation anesthetic especially in pediatric anesthesia, can induce developmental neurotoxicity. In this study, the differentially expressed mRNAs in the hippocampus of newborn rats exposed to 3% sevoflurane for 6 h were detected by RNA-Sequencing. Those data indicated that the mRNA of Klotho was increased after exposure to sevoflurane. Moreover, the protein expression of Klotho was assayed by Western Blot. Besides over-expression and under-expression of Klotho protein, we also detected changes of cell proliferation, ROS, JC-1, and Bcl-2/Bax ratio in PC12 cells exposed to sevoflurane. After exposure to 3% sevoflurane, the expression of Klotho protein increased in the hippocampus of neonatal rats. In PC12 cells, exposure to sevoflurane could increase cellular ROS level, reduce mitochondrial membrane potential and Bcl-2/Bax ratio. While overexpression of Klotho alleviated the above changes, knockdown of Klotho aggravated the injury of sevoflurane. Klotho protein could reduce oxidative stress and mitochondrial injury induced by sevoflurane in the neuron.

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Funding

This study was supported by Guangdong Basic and Applied Basic Research Foundation, Guangdong Province, China (No. 2021A1515011414).

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Author notes

  1. Wan-Yi Lian and Ze-Peng Lu have contributed equally to this work.

Authors and Affiliations

  1. Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, No. 253 Middle Gongye Road, Guangzhou, 510282, Guangdong, People’s Republic of China

    Wan-Yi Lian, Ze-Peng Lu, Wei Zhao, Jia-Qi Zou, Zi-Ying Lu & Hong-Yi Lei

  2. Guangdong-Hongkong-Macau Institute of CNS Regeneration, Ministry of Education CNS Regeneration Collaborative Joint Laboratory, Jinan University, Guangzhou, 510632, People’s Republic of China

    Li-Bing Zhou

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  1. Wan-Yi Lian
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Lian, WY., Lu, ZP., Zhao, W. et al. The Role of Klotho Protein Against Sevoflurane-Induced Neuronal Injury. Neurochem Res 47, 315–326 (2022). https://doi.org/10.1007/s11064-021-03444-5

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