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Transcriptome Sequencing Identifies Potential Biomarker for White Matter Lesions Diagnosis in the Hypertension Population

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Abstract

Hypertension is confirmed to be one of the major risk factors of leukoaraiosis (LA). However, the pathogenesis of LA is not completely understood and there is no reliable indicator for the early diagnosis of LA in the hypertensive population. This study was designed to explore the potential biomarker for LA diagnosis in patients with hypertension. And it serves as the basis for the further study of LA mechanism. In this study, This study included 110 subjects, including 50 in the LA group and 60 in the control group. First, we performed transcriptome sequencing and quantitative PCR (qPCR) in four samples from the LA group, and three from the control group (seven people) to identify relevant long non-coding RNAs (long ncRNAs or lncRNA). The 103 samples were used for qPCR validation of relevant lncRNAs and the results were consistent with the sequencing. In-depth bioinformatics analysis were performed on differentially expressed (DE) lncRNAs and mRNAs. Go-functional enrichment analysis was performed on DE mRNAs. Some DE mRNA were enriched to biological processes associated with LA, And some lncRNAs related to DE mRNAs were traceable through cis/trans analysis, suggesting that they might be regulated in some way. Additionally, potential biomarkers for LA diagnosis in the hypertension population were identified via RT-qPCR and receive operating characteristic curve (ROC) analysis of lncRNA. One lncRNA, AC020928.1, has been demonstrated to be potential biomarkers for LA diagnosis in the hypertension population. The results of the present study indicated that the lncRNA may have an important role in the pathogenesis of LA and may be a novel target for further research. As the relationship between lncRNAs and LA is just beginning to be unraveled, their specific mechanisms require further investigation.

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Funding

This research was supported by The National Natural Science Fund of China (Grant Number 81771263) and The Key Research and Development Program of Shandong Province (Grant Number 2019GSF108030).

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  1. Wendi Wang and Pei Sun contributed equally to this study.

Authors and Affiliations

  1. Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China

    Wendi Wang

  2. Department of Neurology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250021, China

    Pei Sun, Fengyue Han, Chunjuan Wang, Yongxiang Wang, Xiang Wang, Lin Cong & Chuanqiang Qu

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  1. Wendi Wang
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Correspondence to Chuanqiang Qu.

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Written informed consent was obtained from all subjects. All experimental protocols were reviewed and approved by the Ethics Committee of Shandong Provincial Hospital Affiliated to Shandong University (Jinan, China).

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Wang, W., Sun, P., Han, F. et al. Transcriptome Sequencing Identifies Potential Biomarker for White Matter Lesions Diagnosis in the Hypertension Population. Neurochem Res 46, 2079–2088 (2021). https://doi.org/10.1007/s11064-021-03346-6

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