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Gonadotropin-Releasing Hormone Receptor Expression in Human Spinal Cord

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Abstract

The expression of the gonadotrophin-releasing hormone receptor expression on pituitary gonadotrophs in humans is well characterized. In nervous system they have also been found in hippocampi and cerebral cortex. However, gonadotrophin-releasing hormone receptor expression in human spinal cord has not been reported. This study was to analyze the gonadotrophin-releasing hormone receptor expression in human spinal cord by immunohistochemistry, mRNAs by reverse transcriptase polymerase chain reaction, cDNA cloning and Western blot. The results show immunoreactive material to gonadotrophin-releasing hormone receptor in motoneurons of the spinal cord. Further, the study revealed that spinal cord expressed the gonadotrophin-releasing hormone receptor mRNA. The amplicon sequence corresponds to 100% of identity to GenBank. In Western blot, a band of 37 kDa were found in extracts of spinal cord and placenta as a control. In conclusion, human spinal cord expresses gonadotrophin-releasing hormone receptor analyzed through immunohistochemistry, the expression of its mRNA, cloning its cDNA and Western blot analysis. The presence of gonadotrophin-releasing hormone receptor in the spinal cord suggests the possibility of an extrapituitary functional role independent of reproductive system.

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, Center of Basic Sciences, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Col. Ciudad Universitaria, CP 20131, Aguascalientes, AGS, Mexico

    Carmen Díaz-Galindo, Denisse Calderón-Vallejo, Irma Hernández-Jasso, Diego Martínez-Díaz & J. Luis Quintanar

  2. Department of Morphology, Center of Basic Sciences, Universidad Autónoma de Aguascalientes, Aguascalientes, Mexico

    Denisse Calderón-Vallejo & David Ibarra-Martínez

  3. Department of Microbiology, Center of Basic Sciences, Universidad Autónoma de Aguascalientes, Aguascalientes, Mexico

    Daniel Cervantes-García

  4. Department of Chemistry, Center of Basic Sciences, Universidad Autónoma de Aguascalientes, Aguascalientes, Mexico

    Martín Muñoz-Ortega

Authors
  1. Carmen Díaz-Galindo
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  2. Denisse Calderón-Vallejo
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  3. Irma Hernández-Jasso
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  4. Daniel Cervantes-García
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  5. Diego Martínez-Díaz
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  6. David Ibarra-Martínez
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  7. Martín Muñoz-Ortega
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  8. J. Luis Quintanar
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Correspondence to J. Luis Quintanar.

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Díaz-Galindo, C., Calderón-Vallejo, D., Hernández-Jasso, I. et al. Gonadotropin-Releasing Hormone Receptor Expression in Human Spinal Cord. Neurochem Res 46, 165–170 (2021). https://doi.org/10.1007/s11064-020-03178-w

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  • DOI: https://doi.org/10.1007/s11064-020-03178-w

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