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SNHG16 Silencing Inhibits Neuroblastoma Progression by Downregulating HOXA7 via Sponging miR-128-3p

Neurochemical Research volume 45, pages 825–836 (2020)Cite this article

Abstract

Neuroblastoma (NB) is a common intracranial solid tumor with high mortality. Small nucleolar RNA host gene 16 (SNHG16), one of the long noncoding RNAs (lncRNAs), has been reported to be linked to the poor prognosis of NB. However, the mechanisms of SNHG16 in regulating NB progression remain poorly understood. The expression level of SNHG16 was measured by quantitative real time polymerase chain reaction (qRT-PCR). The starBase was employed to predict the interaction of miR-128-3p and SNHG16 or HOXA7, which was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Cell proliferation and apoptosis were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry, respectively. Transwell assay was used to detect cell invasion or migration. The mRNA and protein levels of homeobox protein A7 (HOXA7) were determined by qRT-PCR and western blot, respectively. The levels of SNHG16 and HOXA7 were conspicuously increased in NB tissues and cells, while the expression of miR-128-3p was obviously declined, compared with corresponding normal tissues and cells. SNHG16 silencing inhibited proliferation, migration and invasion and induced apoptosis of NB cells. We identified that SNHG16 directly interacted with miR-128-3p, and miR-128-3p could target the 3′UTR of HOXA7 in NB cells. Simultaneously, miR-128-3p expression was negatively associated with SNHG16 or HOXA7. Further studies indicated that SNHG16 overexpression rescued the effects of miR-128-3p-mediated on inhibiting proliferation, migration, invasion and promoting apoptosis of NB cells. Moreover, SNHG16 could modulate HOXA7 by sponging miR-128-3p in NB cells. Besides, SNHG16 silencing suppressed tumor growth in vivo. Knockdown of SNHG16 impeded proliferation, migration, invasion and induced apoptosis through the SNHG16/miR-128-3p/HOXA7 axis in NB cells.

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  1. Juntao Bao and Shufeng Zhang have contributed equally to this work.

Affiliations

  1. Department of Pediatric Surgery, People’s Hospital of Zhengzhou University (Henan Provincial People’s Hospital), Zhengzhou, 450003, Henan, China

    Juntao Bao, Shufeng Zhang & Qinglei Meng

  2. Department of Hepatobiliary Surgery, People’s Hospital of Zhengzhou University (Henan Provincial People’s Hospital), No. 7 Weiwu Road, Zhengzhou, 450003, Henan, China

    Tao Qin

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  1. Juntao Bao
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11064_2020_2955_MOESM1_ESM.tif

Fig. S1 MiR-128-3p inhibitor promoted NB progression. (A) The level of miR-128-3p in NB cells transfected with anti-miR-NC or anti-miR-128-3p was checked by qRT-PCR (n=3). (B, C) MTT assay was hired to check cell viability (n=3). (D) Flow cytometry was employed to analyze cell apoptosis (n=3). (E, F) Transwell assay was performed to evaluate the abilities of cell migration and invasion (n=3). *P < 0.05. Supplementary file1 (TIF 3239 kb)

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Bao, J., Zhang, S., Meng, Q. et al. SNHG16 Silencing Inhibits Neuroblastoma Progression by Downregulating HOXA7 via Sponging miR-128-3p. Neurochem Res 45, 825–836 (2020). https://doi.org/10.1007/s11064-020-02955-x

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  • DOI: https://doi.org/10.1007/s11064-020-02955-x

Keywords

  • Neuroblastoma (NB)
  • SNHG16
  • Apoptosis
  • miR-128-3p
  • HOXA7