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Up-Regulation of Cdc37 Contributes to Schwann Cell Proliferation and Migration After Sciatic Nerve Crush

Neurochemical Research volume 43, pages 1182–1190 (2018)Cite this article

Abstract

Cell division cycle protein 37 (Cdc37), a molecular chaperone takes part in a series of cellular processes including cell signal transduction, cell cycle progression, cell proliferation, cell motility, oncogenesis and malignant progression. It can not only recruit immature protein kinases to HSP90 but also work alone. Cdc37 was reported to be associated with neurogenesis, neurite outgrowth, axon guidance and myelination. However, the roles of Cdc37 on Schwann cells (SC) after peripheral nerve injury (PNI) remain unknown. In this study, we found that the expression of Cdc37 increased and reached the peak at 1 week after sciatic nerve crush (SNC), which was consistent with that of proliferation cell nuclear antigen. Immunofluorescence verified that Cdc37 co-localized with SC in vivo and in vitro. Intriguingly, Cdc37 protein level was potentiated in the model of TNF-α-induced SC proliferation. Moreover, we found that Cdc37 silencing impaired proliferation of SC in vitro. Moreover, Cdc37 suppression attenuated kinase signaling pathways of Raf–ERK and PI3K/AKT which are crucial cell signaling for SC proliferation. Finally, we found that Cdc37 silencing inhibited SC migration in vitro. In conclusion, we demonstrated that the way Cdc37 contributed to SC proliferation is likely via activating kinase signaling pathways of Raf–ERK and PI3K/AKT, and CDC37 was also involved in SC migration after SNC.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81471258).

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Author notes

  1. Yuxi Liu and Shuyao Wang have contributed equally to this work.

Affiliations

  1. Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, People’s Republic of China

    Yuxi Liu, Dazhi Ding, Zhaohui Yu, Weiwei Sun & Youhua Wang

  2. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Shuyao Wang

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  1. Yuxi Liu
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  2. Shuyao Wang
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  4. Zhaohui Yu
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  6. Youhua Wang
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Correspondence to Youhua Wang.

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Liu, Y., Wang, S., Ding, D. et al. Up-Regulation of Cdc37 Contributes to Schwann Cell Proliferation and Migration After Sciatic Nerve Crush. Neurochem Res 43, 1182–1190 (2018). https://doi.org/10.1007/s11064-018-2535-6

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  • DOI: https://doi.org/10.1007/s11064-018-2535-6

Keywords

  • Cdc37
  • Sciatic nerve crush
  • Schwann cell
  • Proliferation
  • Signaling pathways
  • Migration