MiR-183-5p Alleviates Chronic Constriction Injury-Induced Neuropathic Pain Through Inhibition of TREK-1
MicroRNAs have been implicated in nerve injury and neuropathic pain. In the previous study we had shown that miR-96 can attenuate neuropathic pain through inhibition of Nav1.3. In this study, we investigated the role of miR-183, a same cluster member of microRNA with miR-96, in neuropathic pain and its potential mechanisms. We found that the expression level of miR-183-5p in dorsal root ganglion was decreased with the development of neuropathic pain induced by chronic constriction sciatic nerve injury (CCI). By contrast, the TREK-1, a K+ channel, was increased. Further investigation identified that intrathecal injection of miR-183-5p mimic efficiently ameliorated neuropathic pain and inhibited the expression of TREK-1, a predicted target gene of miR-183-5p. Luciferase assays confirmed the binding of miR-183-5p and TREK-1. In addition, over-expression of TREK-1 blocked the roles of miR-183-5p in neuropathic pain. Our findings suggested that miR-183-5P participated in the regulation of CCI-induced neuropathic pain through inhibiting the expression of TREK-1.
KeywordsMiR-183-5p TREK-1 Chronic constriction injury Neuropathic pain Dorsal root ganglion
This study was supported by the National Natural Science Foundation of China (Nos. 31760274, 81760213, 81260318), Outstanding Young Talent Support Plan of Jiangxi Province (No. 20171BCB23027), and Natural Science Foundation of Jiangxi Province (No. 20171BAB204017).
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflicts of interests.
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