Gualou Guizhi Granule Protects Against Oxidative Injury by Activating Nrf2/ARE Pathway in Rats and PC12 Cells

Original Paper
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Abstract

Stroke involves numerous pathophysiological processes and oxidative stress is considered as a main cellular event in its pathogenesis. The nuclear factor erythroid-2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway plays a key role in inducing phase II detoxifying enzymes and antioxidant proteins and is now considered as a interesting therapeutic target for the treatment of stroke. The objective of this study is to investigate the protective effect of Gualou Guizhi granule (GLGZG) against oxidative stress and explore the protective mechanism of the Nrf2/ARE pathway. In vivo, administration of GLGZG in a rat model of focal cerebral ischemia significantly suppressed oxidative injury by increasing the activity of superoxide dismutase and glutathione level and decreasing reactive oxygen species and malondialdehyde levels. Western blot analysis showed that GLGZG induced nuclear translocation of Nrf2, and combined with real-time PCR results, which indicated that GLGZG up-regulated the Nrf2/ARE pathway. In addition, in cultured PC12 cells, GLGZG protected against H2O2 induced oxidative injury and activated the Nrf2/ARE pathway. All the results demonstrated that GLGZG in the management of cerebral ischemia and H2O2 induced oxidative injury may be associated with activation of Nrf2/ARE signaling pathway.

Keywords

Gualou Guizhi granule Cerebral ischemia Oxidative stress Nrf2 

Notes

Acknowledgements

This research was done in Collaborative Innovation Center for Rehabilitation Technology and TCM Rehabilitation Research Center of SATCM and was funded by the National Natural Science Foundation of China (No. 81674046) and Fujian Provincial Health and Family Planning Commission Youth Talent Project (2017-1-71).

Compliance with Ethical Standards

Conflict of interest

All the authors declare no conflict of interest.

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Authors and Affiliations

  1. 1.College of PharmacyFujian University of Traditional Chinese MedicineFuzhouPeople’s Republic of China
  2. 2.State Key Laboratory of Chinese Pharmacies of Fujian Provincial Department of Science and TechnologyFujian University of Traditional Chinese MedicineFuzhouPeople’s Republic of China

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