Rifampicin Prevents SH-SY5Y Cells from Rotenone-Induced Apoptosis via the PI3K/Akt/GSK-3β/CREB Signaling Pathway
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In addition to its original application for treating tuberculosis, rifampicin has multiple potential neuroprotective effects in chronic neurodegenerative diseases including Parkinson’s disease (PD) and Alzheimer’s disease. Inflammatory reactions and the PI3K/Akt pathway are strongly implicated in dopaminergic neuronal death in PD. This study aims to investigate whether rifampicin protects rotenone-lesioned SH-SY5Y cells via regulating PI3K/Akt/GSK-3β/CREB pathway. Rotenone-treated SH-SY5Y cells were used as the cell model to investigate the neuroprotective effects of rifampicin. Cell viability and apoptosis of SH-SY5Y cells were determined by CCK-8 assay and flow cytometry, respectively. The expression of Akt, p-Akt, GSK-3β, p-GSK-3β, CREB and p-CREB were measured by Western blot. Our results showed that the cell viability and level of phospho-CREB significantly decreased in SH-SY5Y cells exposed to rotenone when compared to the control group. Both the cell viability and the expression of phospho-CREB in cells pretreated with rifampicin were higher than those of cells exposed to rotenone alone. Moreover, pretreatment of SH-SY5Y cells with rifampicin enhanced phosphorylation of Akt and suppressed activity of GSK-3β. The addition of LY294002, a PI3K inhibitor, could suppress phosphorylation of Akt and CREB and activate GSK-3β, resulting in abolishment of neuroprotective effects of rifampicin on cells exposed to rotenone. Rifampicin provides neuroprotection against dopaminergic degeneration, partially via the PI3K/Akt/GSK-3β/CREB signaling pathway. These findings suggest that rifampicin could be an effective and promising neuroprotective candidate for treating PD.
KeywordsRifampicin Neuroprotection Rotenone Glycogen synthase kinase-3β cAMP response element-binding protein
This work was supported by grants from the National Natural Science Foundation of China (Grant Numbers 81571244, 81771378, 81371391 and 81503052), the Public Welfare Research and Capacity Building Project of Guangdong Province (Grant Number 2014A020212164), the Natural Science Foundation of Guangdong Province (Grant Numbers 2014A030313202, 2016A030313322 and 2017A030313840), the Guangzhou Science and Technology Research Project (Grant Number 201607010219), and the Fundamental Research Fund for University Youth Scholars (Grant Number 17ykpy39).
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Conflict of interest
All authors announce no competing interests.
For this type of study formal consent is not required.
Research Involving Human and Animal Participants
The article does not contain any studies with human participants or animals performed by any of the authors.
- 7.Mori N, Fujii M, Cheng G, Ikeda S, Yamasaki Y, Yamada Y, Tomonaga M, Yamamoto N (2001) Human T-cell leukemia virus type I tax protein induces the expression of anti-apoptotic gene Bcl-xL in human T-cells through nuclear factor-kappaB and c-AMP responsive element binding protein pathways. Virus Genes 22:279–287CrossRefPubMedGoogle Scholar
- 17.Ramkumar M, Rajasankar S, Gobi VV, Dhanalakshmi C, Manivasagam T, Justin Thenmozhi A, Essa MM, Kalandar A, Chidambaram R (2017) Neuroprotective effect of Demethoxycurcumin, a natural derivative of Curcumin on rotenone induced neurotoxicity in SH-SY 5Y Neuroblastoma cells. BMC Complement Altern Med 17:217CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Lin D, Jing X, Chen Y, Liang Y, Lei M, Peng S, Zhou T, Zheng D, Zeng Z, Wu X, Yang L, Xiao S, Liu J, Tao E (2017) Rifampicin pre-treatment inhibits the toxicity of rotenone-induced PC12 cells by enhancing sumoylation modification of alpha-synuclein. Biochem Biophys Res Commun 485:23–29CrossRefPubMedGoogle Scholar
- 21.Lin R, Lin Y, Tao J, Chen B, Yu K, Chen J, Li X, Chen LD (2015) Electroacupuncture ameliorates learning and memory in rats with cerebral ischemia-reperfusion injury by inhibiting oxidative stress and promoting p-CREB expression in the hippocampus. Mol Med Rep 12:6807–6814CrossRefPubMedGoogle Scholar
- 23.Credle JJ, George JL, Wills J, Duka V, Shah K, Lee YC, Rodriguez O, Simkins T, Winter M, Moechars D, Steckler T, Goudreau J, Finkelstein DI, Sidhu A (2015) GSK-3beta dysregulation contributes to parkinson’s-like pathophysiology with associated region-specific phosphorylation and accumulation of tau and alpha-synuclein. Cell Death Differ 22:838–851CrossRefPubMedGoogle Scholar