Neurochemical Research

, Volume 43, Issue 4, pp 869–877 | Cite as

Analgesic Effect of Methane Rich Saline in a Rat Model of Chronic Inflammatory Pain

  • Shu-Zhuan Zhou
  • Ya-Lan Zhou
  • Feng Ji
  • Hao-Ling Li
  • Hu Lv
  • Yan Zhang
  • Hua Xu
Original Paper


How oxidative stress contributes to neuro-inflammation and chronic pain is documented, and methane is reported to protect against ischemia–reperfusion injury in the nervous system via anti-inflammatory and antioxidant properties. We studied whether methane in the form of methane rich saline (MS) has analgesic effects in a monoarthritis (MA) rat model of chronic inflammatory pain. Single and repeated injections of MS (i.p.) reduced MA-induced mechanical allodynia and multiple methane treatments blocked activation of glial cells, decreased IL-1β and TNF-α production and MMP-2 activity, and upregulated IL-10 expression in the spinal cord on day 10 post-MA. Furthermore, MS reduced infiltrating T cells and expression of IFN-γ and suppressed MA-induced oxidative stress (MDA and 8-OHDG), and increased superoxide dismutase and catalase activity. Thus, MS may offer anti-inflammatory and antioxidant effects to reduce chronic inflammatory pain.


Chronic inflammatory pain Oxidative stress T cells Glial cell Neuro-inflammation Methane rich saline 



This study was supported by the Science and Technology Commission of Shanghai Municipality, China (STCSM, 16ZR1400500).

Author Contributions

S-ZZ, YLZ and FJ performed the behavioral tests, western blot, flow cytometry, gelatin zymographic analysis and drafted the manuscript. HLL and HL participated in the oxidative stress assays, immunohistochemistry, ELISA experiments. YZ produced the statistical analysis. HX conceived the study, designed the experiments, wrote the paper. All of the authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they do not have any conflicts of interest.

Supplementary material

11064_2018_2490_MOESM1_ESM.tif (246.4 mb)
Supplementary material 1 (TIF 252364 KB)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of AnesthesiologyChanghai Hospital, The Second Military Medical UniversityShanghaiChina
  2. 2.Department of Anesthesiology188 Hospital of PLAChaozhouChina
  3. 3.Department of Anesthesiology, Shuguang HospitalShanghai University of Traditional Chinese MedicineShanghaiChina
  4. 4.Department of AnesthesiologyThe First Affiliated Hospital of Zhejiang UniversityHangzhouChina
  5. 5.Department of AnesthesiologyFudan University Shanghai Cancer CenterShanghaiChina

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