Neurochemical Research

, Volume 42, Issue 4, pp 1084–1095 | Cite as

Upregulated Expression of TRIM32 Is Involved in Schwann Cell Differentiation, Migration and Neurite Outgrowth After Sciatic Nerve Crush

  • Yonghua Liu
  • Weijie Wu
  • Huiguang Yang
  • Zhengming Zhou
  • Xiaojian Zhu
  • Chi Sun
  • Yuxi Liu
  • Zhaohui Yu
  • Yuyan Chen
  • Youhua WangEmail author
Original Paper


Tripartite motif containing 32 (TRIM32), a member of the tripartite motif (TRIM) family, plays an indispensable role in myoblast proliferation. It also regulates neuron and skeletal muscle stem cell differentiation. Although it is of great importance, we know little about the roles of TRIM32 during peripheral nervous system injury. Here, we examined the dynamic changes of TRIM32 in acute sciatic nerve crush (SNC) model. After crush, TRIM32 rapidly increased and reached the climax at 1 week but then gradually declined to the normal level at 4 weeks post-injury. Meanwhile, we observed similar changes of Oct-6. What is more, we found co-localization of TRIM32 with S100 and Oct-6 in 1-week-injured tissues using double immunofluorescent staining. In further vitro experiments, enhancive expression of TRIM32 was detected during the process of cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation and nerve growth factor (NGF)-induced PC12 cell neurite outgrowth. More interestingly, specific si-TRIM32-transfected RSC96 cells exhibited obvious reduction in the ability of migration. Taken together, we inferred that upregulated TRIM32 was not only involved in the differentiation and migration of Schwann cells but the neurite elongation after SNC.


TRIM32 Schwann cell Differentiation PC12 cell Neurite outgrowth Sciatic nerve crush 



This work was supported by the National Natural Science Foundation of China (No. 81471258, No. 31300902) and the Applied Basic Research Project of Nantong City (No. MS12015099).

Compliance with Ethical Standards

Conflict of interest



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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yonghua Liu
    • 1
    • 2
    • 3
  • Weijie Wu
    • 2
  • Huiguang Yang
    • 4
  • Zhengming Zhou
    • 4
  • Xiaojian Zhu
    • 5
  • Chi Sun
    • 2
  • Yuxi Liu
    • 2
  • Zhaohui Yu
    • 2
  • Yuyan Chen
    • 6
  • Youhua Wang
    • 2
    Email author
  1. 1.Jiangsu Province Key Laboratory for Inflammation and Molecular Drug TargetNantong UniversityNantongPeople’s Republic of China
  2. 2.Department of Orthopaedics, Affiliated Hospital of Nantong UniversityNantong UniversityNantongPeople’s Republic of China
  3. 3.Department of Pathogen Biology, Medical CollegeNantong UniversityNantongPeople’s Republic of China
  4. 4.Department of Orthopaedics, Affiliated Jiangyin Hospital of Nantong UniversityNantong UniversityNantongPeople’s Republic of China
  5. 5.Department of Orthopaedics, Affiliated Mental Health Center of Nantong UniversityNantong UniversityNantongPeople’s Republic of China
  6. 6.Class 2, Grade 13, Clinical Medicine, Medical CollegeNantong UniversityNantongPeople’s Republic of China

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