Neurochemical Research

, Volume 42, Issue 2, pp 606–614 | Cite as

EGb-761 Attenuates the Anti-proliferative Activity of Fluoride via DDK1 in PC-12 Cells

  • Cai-Yi Zhang
  • Rui Chen
  • Fen Wang
  • Chao Ren
  • Peng Zhang
  • Qian Li
  • Hui-Hua Li
  • Ke-Tai Guo
  • De-Qin Geng
  • Chun-feng LiuEmail author
Original Paper


EGb-761 is commonly used as a treatment for ischemic brain injury, neurodegenerative diseases and some types of tumors (Christen and Maixent, in Cell Mol Biol 48(6):601–611, 2002). However, it is unclear whether EGb-761 affects the proliferation of cells exposed to fluoride. In this study, the proliferation and apoptosis of PC-12 cells exposed to fluoride were investigated and EGb-761 was used to protect PC-12 cells against the effects of fluoride. We found that the canonical Wnt signaling pathway was involved in the anti-proliferation of PC-12 cells exposed to fluoride. Furthermore, the results also showed that EGb-761 could attenuate the anti-proliferative activity of fluoride via DDK1 in PC-12 cells. This study may provide a new method for protecting against the inhibition of cell proliferation induced by fluoride.


EGb-761 DKK1 Wnt singaling pathway Proliferation 



This study was supported by BK20151159 from the Natural Science Foundation of Jiangsu Province, China, Project 81501185 of National Natural Science Foundation of China, Xuzhou Medical Talents Project and Xuzhou technological and scientific project No. KC14SH50.

Compliance with Ethical Standards

Conflict of interest

There is no conflict of interest to disclose.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Cai-Yi Zhang
    • 1
    • 2
  • Rui Chen
    • 3
  • Fen Wang
    • 1
    • 4
  • Chao Ren
    • 5
  • Peng Zhang
    • 2
  • Qian Li
    • 1
  • Hui-Hua Li
    • 3
  • Ke-Tai Guo
    • 4
  • De-Qin Geng
    • 3
  • Chun-feng Liu
    • 1
    • 4
    Email author
  1. 1.Department of Neurology and Suzhou Clinical Research Center of Neurological DiseaseThe Second Affiliated Hospital of Soochow UniversitySuzhouChina
  2. 2.Xuzhou Medical University Affiliated Xuzhou Oriental HospitalXuzhouChina
  3. 3.Department of NeurologyXuzhou Medical University Affiliated HospitalXuzhouChina
  4. 4.Institute of NeuroscienceSoochow UniversitySuzhouChina
  5. 5.Department of NeurologyAffiliated Yantai Yuhuangding Hospital of Qingdao University Medical CollegeYantaiChina

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