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(−)-Epigallocatechin Gallate Inhibits Asymmetric Dimethylarginine-Induced Injury in Human Brain Microvascular Endothelial Cells

Abstract

(−)-Epigallocatechin gallate (EGCG) is the main polyphenol component of green tea (leaves of the Camellia sinensis plant). EGCG has been reported to protect human brain microvascular endothelial cells (HBMECs) against injury in several models. However, the exact mechanism is still unclear. In the current study we found that EGCG protected against asymmetric dimethylarginine (ADMA)-induced HBMEC injury, and inhibited ADMA-induced reactive oxygen species production and malondialdehyde expression. At the same time, we found that pretreatment with EGCG attenuated the upregulation of Bax and the downregulation of Bcl-2, thus confirming the cellular protective properties of EGCG against ADMA-induced apoptosis. Furthermore, we found that EGCG inhibited ADMA-induced phosphorylation of ERK1/2 and p-38, whose inhibitors relieved HBMEC injury. In conclusion, EGCG can protect against ADMA-induced HBMEC injury via the ERK1/2 and p38 MAPK pathways, which are involved in the underlying mechanisms of HBMEC injury in cerebral infarction.

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Correspondence to Changlin Zou.

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Jia Li and Changlin Zou contributed equally to this work as the co-first author.

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Li, J., Zhang, Z., Lv, L. et al. (−)-Epigallocatechin Gallate Inhibits Asymmetric Dimethylarginine-Induced Injury in Human Brain Microvascular Endothelial Cells. Neurochem Res 41, 1868–1876 (2016). https://doi.org/10.1007/s11064-016-1898-9

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  • DOI: https://doi.org/10.1007/s11064-016-1898-9

Keywords

  • Cerebral infarction
  • (−)-Epigallocatechin gallate
  • Human brain microvascular endothelial cells
  • Asymmetric dimethylarginine
  • Mitogen-activated protein kinases