Neurochemical Research

, Volume 41, Issue 5, pp 1035–1041 | Cite as

Combined Low-Intensity Exercise and Ascorbic Acid Attenuates Kainic Acid-Induced Seizure and Oxidative Stress in Mice

  • Hee-jae Kim
  • Wook Song
  • Eun Hee Jin
  • Jongkyu Kim
  • Yoonseok Chun
  • Eung Nam An
  • Sok ParkEmail author
Original Paper


Physical exercise and vitamins such as ascorbic acid (ASC) have been recognized as an effective strategy in neuroprotection and neurorehabilitatioin. However, there is a need to find an efficient treatment regimen that includes ASC and low-intensity exercise to diminish the risk of overtraining and nutritional treatment by attenuating oxidative stress. In the present study, we investigated the combined effect of low-intensity physical exercise (EX) and ASC on kainic acid (KA)-induced seizure activity and oxidative stress in mice. The mice were randomly assigned into groups as follows: “KA only” (n = 11), “ASC + KA” (n = 11), “Ex + KA” (n = 11), “ASC + Ex + KA” (n = 11). In the present study, low intensity of swimming training period lasted 8 weeks and consisted of 30-min sessions daily (three times per week) without tail weighting. Although no preventive effect of low-intensity exercise or ASC on KA seizure occurrence was evident, there was a decrease of seizure activity, seizure development (latency to first seizures), and mortality in “ASC + Ex + KA” compared to “ASC + KA”, “Ex + KA”, and “KA only” group. In addition, a preventive synergistic coordination of low-intensity exercise and ASC was evident in glutathione peroxidase and superoxide dismutase activity compared to separate treatment. These results suggest that low-intensity exercise and ASC treatment have preventive effects on seizure activity and development with alternation of oxidative status.


Seizure activity Ascorbic acid Low-intensity swimming Kainic acid Oxidative stress 



This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology, MEST (2011-0030135), (NRF-2013M3A9B6046417), (2014S1A5A8018765).


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Hee-jae Kim
    • 1
  • Wook Song
    • 1
    • 2
  • Eun Hee Jin
    • 3
  • Jongkyu Kim
    • 4
  • Yoonseok Chun
    • 5
  • Eung Nam An
    • 3
  • Sok Park
    • 6
    Email author
  1. 1.Health and Exercise Science Laboratory, Institute of Sports ScienceSeoul National UniversitySeoulKorea
  2. 2.Institute on AgingSeoul National UniversitySeoulKorea
  3. 3.Department of Sports ScienceSungkyunkwan UniversitySeoulKorea
  4. 4.Aribio Sports Science InstituteYong In UniversityYonginKorea
  5. 5.Sports Wellness CenterYong In UniversityYonginKorea
  6. 6.Division of Sports Industry and Science, Department of Sports and Health ManagementMokwon UniversityTaejonKorea

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