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Mitochondrial Respiration Chain Enzymatic Activities in the Human Brain: Methodological Implications for Tissue Sampling and Storage

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Abstract

Mitochondrial respiratory chain complexes enzymatic (MRCCE) activities were successfully evaluated in frozen brain samples. Epilepsy surgery offers an ethical opportunity to study human brain tissue surgically removed to treat drug resistant epilepsies. Epilepsy surgeries are done with hemodynamic and laboratory parameters to maintain physiology, but there are no studies analyzing the association among these parameters and MRCCE activities in the human brain tissue. We determined the intra-operative parameters independently associated with MRCCE activities in middle temporal neocortex (Cx), amygdala (AMY) and head of hippocampus (HIP) samples of patients (n = 23) who underwent temporal lobectomy using multiple linear regressions. MRCCE activities in Cx, AMY and HIP are differentially associated to trans-operative mean arterial blood pressure, O2 saturation, hemoglobin, and anesthesia duration to time of tissue sampling. The time-course between the last seizure occurrence and tissue sampling as well as the sample storage to biochemical assessments were also associated with enzyme activities. Linear regression models including these variables explain 13–17 % of MRCCE activities and show a moderate to strong effect (r = 0.37–0.82). Intraoperative hemodynamic and laboratory parameters as well as the time from last seizure to tissue sampling and storage time are associated with MRCCE activities in human samples from the Cx, AMYG and HIP. Careful control of these parameters is required to minimize confounding biases in studies using human brain samples collected from elective neurosurgery.

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Acknowledgments

This work was supported by FAPESC/CNPq, Programa de Apoio a Núcleos de Excelência PRONEX (NENASC Project), Santa Catarina, Brazil.

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    Authors and Affiliations

    1. Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil

      Marcelo Fernando Ronsoni, Alexandre Hohl, Iris H. Z. Troncoso, Rodrigo Bainy Leal, Charles Kondageski, Jean Costa Nunes, Marcelo Neves Linhares, Kátia Lin, Alexandra Susana Latini & Roger Walz

    2. Laboratório de Bioenergética e Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil

      Aline Pertile Remor & Alexandra Susana Latini

    3. Laboratório de Transdução de Sinal no Sistema Nervoso Central, Departamento de Bioquímica, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil

      Mark William Lopes & Rodrigo Bainy Leal

    4. Centro de Ensino e Treinamento Integrado de Anestesiologia, Hospital Governador Celso Ramos (HGCR), Florianópolis, SC, Brazil

      Gustavo Luchi Boos

    5. Laboratório de Neuropatologia, Serviço de Patologia, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil

      Jean Costa Nunes

    6. Serviço de Cirurgia de Epilepsia, Hospital Governador Celso Ramos (HGCR), Florianópolis, SC, Brazil

      Marcelo Neves Linhares

    7. Divisão de Neurocirurgia, Departamento de Cirurgia, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil

      Charles Kondageski

    8. Serviço de Neurologia, Departamento de Clínica Médica, Hospital Universitário, 3 andar, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, 88.040-970, Brazil

      Kátia Lin & Roger Walz

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    1. Marcelo Fernando Ronsoni
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    2. Aline Pertile Remor
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    3. Mark William Lopes
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    4. Alexandre Hohl
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    10. Marcelo Neves Linhares
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    13. Roger Walz
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    Correspondence to Roger Walz.

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    The authors declare that there is no conflict of interest in the study.

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    Marcelo Fernando Ronsoni and Aline Pertile Remor have contributed equally to this work.

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    Ronsoni, M.F., Remor, A.P., Lopes, M.W. et al. Mitochondrial Respiration Chain Enzymatic Activities in the Human Brain: Methodological Implications for Tissue Sampling and Storage. Neurochem Res 41, 880–891 (2016). https://doi.org/10.1007/s11064-015-1769-9

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    • DOI: https://doi.org/10.1007/s11064-015-1769-9

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