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Effects of Ferrofluid and Phytoalexin Spirobrassinin on Thioflavin-T-Based Fluorescence in Cerebrospinal Fluid of the Elderly and Multiple Sclerosis Patients

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Abstract

It is well known that misfolded peptides/proteins can play a role in processes of normal ageing and in the pathogenesis of many diseases including Alzheimer’s disease. Previously, we evaluated samples of cerebrospinal fluid from patients with Alzheimer’s disease and multiple sclerosis by means of thioflavin-T-based fluorescence. We observed attenuated effects of magnetite nanoparticles operated via anti-aggregation actions on peptides/proteins from patients with Alzheimer’s disease but not from those with multiple sclerosis when compared to age-related controls. In this study, we have evaluated the in vitro effects of anti-aggregation operating ferrofluid and phytoalexin spirobrassinin in the cerebrospinal fluid of patients with multiple sclerosis and Alzheimer’s disease. We have found significant differences in native fluorescence (λ excitation = 440 nm, λ emission = 485 nm) of samples among particular groups (young controls < multiple sclerosis, Alzheimer’s disease < old controls). Differences among groups were observed also in thioflavin-T-based fluorescence (young controls = multiple sclerosis < Alzheimer’s disease < old controls) and the most marked change from native to thioflavin-T-based fluorescence was found in young controls (28–40 years old people). Both ferrofluid and spirobrassinin evoked drops in thioflavin-T-based fluorescence; however, ferrofluid was more efficient in old controls (54–75 years old people) and spirobrassinin in multiple sclerosis patients, both compared to young controls. The results are discussed especially in relation to aggregated peptides/proteins and liposoluble fluorescent products of lipid peroxidation. Based on the significant effect of spirobrassinin in vitro, we suggest that spirobrassinin may be of therapeutic value in multiple sclerosis.

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Acknowledgments

In Slovakia, the study was supported within the projects Nos. 26220120021, 26220220005 in frame of Structural Funds of European Union, VEGA 0181 and projects of the Slovak Research and Development Agency, contract nos. APVV-0171-10 and SK-RO-0016-12. In the Czech Republic, the study was supported by IGA NT 13183, GACR P304/12/G069 and MH CZ-DRO (PCP, 00023752) project. The authors thank M. Koneracka and V. Zavisova for preparing the ferrofluid and P. Kutschy for synthesis of and providing the spirobrassinin.

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Authors and Affiliations

  1. Prague Psychiatric Centre, Alzheimer Disease Centre, Ustavni 91, 181 03, Prague 8–Bohnice, Czech Republic

    Zdena Kristofikova, Ales Bartos, Jan Ricny, Jana Sirova & Daniela Ripova

  2. Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01, Kosice, Slovakia

    Zuzana Gazova & Katarina Siposova

  3. Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine, Safarik University, Trieda SNP 1, 040 11, Kosice, Slovakia

    Zuzana Gazova

  4. Department of Neurology, Third Faculty of Medicine, University Hospital Kralovske Vinohrady, Charles University in Prague, Srobarova 50, 100 34, Prague 10, Czech Republic

    Ales Bartos & Jolana Kotoucova

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  1. Zdena Kristofikova
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  2. Zuzana Gazova
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  3. Katarina Siposova
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Correspondence to Zdena Kristofikova.

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Kristofikova, Z., Gazova, Z., Siposova, K. et al. Effects of Ferrofluid and Phytoalexin Spirobrassinin on Thioflavin-T-Based Fluorescence in Cerebrospinal Fluid of the Elderly and Multiple Sclerosis Patients. Neurochem Res 39, 1502–1510 (2014). https://doi.org/10.1007/s11064-014-1340-0

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  • DOI: https://doi.org/10.1007/s11064-014-1340-0

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