Antioxidant Effects of Nerolidol in Mice Hippocampus After Open Field Test
- 884 Downloads
The aim of this study was to evaluate the neuroprotective effects of nerolidol in mice hippocampus against oxidative stress in neuronal cells compared to ascorbic acid (positive control) as well as evaluated the nerolidol sedative effects by open field test compared to diazepam (positive control). Thirty minutes prior to behavioral observation on open field test, mice were intraperitoneally treated with vehicle, nerolidol (25, 50 and 75 mg/kg), diazepam (1 mg/kg) or ascorbic acid (250 mg/kg). To clarify the action mechanism of of nerolidol on oxidative stress in animals subjected to the open field test, Western blot analysis of Mn-superoxide dismutase and catalase in mice hippocampus were performed. In nerolidol group, there was a significant decrease in lipid peroxidation and nitrite levels when compared to negative control (vehicle). However, a significant increase was observed in superoxide dismutase and catalase activities in this group when compared to the other groups. Vehicle, diazepam, ascorbic acid and nerolidol groups did not affected Mn-superoxide dismutase, catalase mRNA or protein levels. Our findings strongly support the hypothesis that oxidative stress occurs in hippocampus. Nerolidol showed sedative effects in animals subjected to the open field test. Oxidative process plays a crucial role on neuronal pathological consequence, and implies that antioxidant effects could be achieved using this sesquiterpene.
KeywordsHippocampus Essential oils Nerolidol Oxidative stress Open field test
This work was supported in part by Grants from the Brazilian National Research Council (CNPq), Brazil. R.M.F and P.S.S. are fellows from CNPq.
- 8.Ferreira PMP, Carvalho AFFU, Sousa DF, Magalhães JF, Martins AR, Martins MAC, Queiroz MGR (2007) Water extract of Moringa oleifera seeds: a toxicological approach. Rev Eletr Pesq Med 1:45–57Google Scholar
- 16.Klopell FC, Lemos M, Sousa JP, Comunello E, Maistro EL, Bastos JK, Andrade SF (2007) Nerolidol, an antiulcer constituent from the essential oil of Baccharis dracunculifolia DC (Asteraceae). Z Naturforsch 62:537–542Google Scholar
- 19.IFRA (International Fragrance Association) (2004) Use level surveyGoogle Scholar
- 20.IFRA (International Fragrance Association) (2007) Volume of use surveyGoogle Scholar
- 22.Nogueira Neto JD, Almeida AAC, Silva OA, Carvalho RBF, De Sousa DP, Freitas RM (2012) Avaliação da toxicidade aguda e das propriedades ansiolíticas do nerolidol em camundongos. Biofar Revista de Biologia e Farmácia 8:42–55Google Scholar
- 24.Nogueira Neto JD, De Sousa DP, Freitas RM (2013) Evaluation of antioxidant potential in vitro of nerolidol. J Basic Appl Pharma Sci 34:125–130Google Scholar
- 43.Hollis L, Jones RS (2009) US Environmental Protection Agency Office of Pesticide Programs. Biopesticides and Pollution Prevention Division, Farnesol Nerolidol 1:24Google Scholar
- 46.Chirayu D, Pandya KR, Howell AP (2012) Antioxidants as potential therapeutics for neuropsychiatric disorders. Prog Neuropsychopharmacol Biol Psychiatry (in press)Google Scholar
- 51.Janowska B, Kurpios-Piec D, Prorok P, Szparecki G, Komisarski M, Kowalczyk P, Janion C, Tudek B (2012) Role of damage-specific DNA polymerases in M13 phage mutagenesis induced by a major lipid peroxidation product trans-4-hydroxy-2-nonenal. Mutat Res 29:41–51Google Scholar
- 53.Halliwell B, Gutteridge JCM (1999) Free radicals in biology and medicine, 3rd edn. Oxford Science Publications, LondonGoogle Scholar
- 55.Yoshida WB, Tardini DMS (2003) Brain injury due to ischemia and reperfusion in carotid edndarterectomy surgery. J Vasc Br 2:119–128Google Scholar