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The Protective Effects of Achyranthes bidentata Polypeptides on Rat Sciatic Nerve Crush Injury Causes Modulation of Neurotrophic Factors

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Abstract

Pharmacological treatment is a therapeutic approach to improving nerve regeneration and functional recovery after peripheral nerve crush injury. The objective of the present study was to investigate the effects of the polypeptides isolated from Achyranthes bidentata Blume (abbreviated as ABPP) on rat sciatic crush injury and to test the possible involvement of neurotrophic factors. After surgical crush injury, rats received daily intraperitoneal injection of 0.2 ml saline containing 2 mg ABPP, 1 μg nerve growth factor (NGF) or no additive. The results from walking track analysis, electrophysiological assessment and histological evaluation indicated that the repair outcomes by ABPP treatment were close to those by NGF treatment, but better than those by treatment with saline alone. The quantitative real-time RT-PCR was used to monitor the mRNA expression of growth associated protein in the crush nerves and the mRNA expression of NGF, brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), tyrosine kinase (Trk)A and TrkB in the dorsal root ganglia (DRGs) at L4–L6. The mRNA expression of these genes in the crush nerve sample and DRGs sample was higher after treatment with ABPP or NGF than after treatment with saline alone. Our findings suggest that ABPP might protect peripheral nerve against crush injury through stimulating release of neurotrophic factors and the other cytokines.

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Acknowledgments

The financial supports of National Natural Science Foundation of China (Grant No. 30970996), “Liu Da Ren Cai Gao Feng” of Jiangsu province (Grant No. Human Resources and Social Security Department in Jiangsu Province (2010) No. 555), and “333 Ren Cai Gong Cheng” of Jiangsu province (2011, III-2240) “226 Ren Cai Gong Cheng” of Nantong are gratefully acknowledged. We thank Professor Jie Liu for assistant in manuscript preparation.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, China

    Yaxian Wang, Lingyan Yang, Hualong zhao, Wei Gu & Ying Yuan

  2. Medical School, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, China

    Weixing Shen

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  1. Yaxian Wang
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  2. Weixing Shen
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  3. Lingyan Yang
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  4. Hualong zhao
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  5. Wei Gu
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Correspondence to Ying Yuan.

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Yaxian Wang and Weixing Shen contributed equally to this work.

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Wang, Y., Shen, W., Yang, L. et al. The Protective Effects of Achyranthes bidentata Polypeptides on Rat Sciatic Nerve Crush Injury Causes Modulation of Neurotrophic Factors. Neurochem Res 38, 538–546 (2013). https://doi.org/10.1007/s11064-012-0946-3

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  • DOI: https://doi.org/10.1007/s11064-012-0946-3

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