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Neuroprotective potential of epigallo catechin-3-gallate in PC-12 cells

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Abstract

Oxidative stress is a major player in aging and neurodegenerative disorders. Macromolecular damage occurs as a result of oxidative stress that affects the mitochondria. Mitochondrial damage leads to cell death by apoptosis or necrosis. EGCG is a tea polyphenol that protects the cells against oxidative stress. Neuroprotective potential of EGCG was tested against H2O2 induced oxidative stress in PC-12 cells. PC-12 cells were grown in tissue culture flasks. Oxidative stress was induced by adding H2O2 to the cells. EGCG was also added and the cell death was assessed using MTT assay. Oxidative stress was assessed by protein carbonyl and thiol status. Mitochondrial membrane potential was studied using JC-1 staining. TNF-α levels were assessed using ELISA. H2O2 increased the protein carbonyl content and reduced the thiol status in the PC-12 cells. Cell death was increased in H2O2 treated cells as shown by MTT assay. Mitochondrial membrane potential was also decreased along with increase in TNF-α level in H2O2 treated cells. EGCG brought about an increase in the cellular thiol status and decreased the protein carbonyl content in the PC-12 cells. Cell death was attenuated by EGCG treatment along with an increase in mitochondrial membrane potential and decrease in TNF-α level. EGCG conferred its antioxidant potential to PC-12 cells as evident by decreased protein damage. Mitochondrial membrane potential was improved along with a decrement in the cell death in PC-12 cells. EGCG acts as a good neutraceutical antioxidant to render neuroprotectivity to PC-12 cells.

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Acknowledgments

The present study was financially supported by JRF and SRF from Indian Council of Medical Research (ICMR), New Delhi, Government of India.

Conflict of interest

The authors wish to state that “No competing financial interests exist” regarding this publication.

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Authors and Affiliations

  1. Department of Genetic Engineering, SRM University, Kattankulathur, 603203, Tamil Nadu, India

    Ravichandran Srividhya

  2. Department of Medical Biochemistry, Dr. ALM. PGIBMS, University of Madras, Chennai, 600 113, Tamil Nadu, India

    Periandavan Kalaiselvi

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  1. Ravichandran Srividhya
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  2. Periandavan Kalaiselvi
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Correspondence to Ravichandran Srividhya.

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Srividhya, R., Kalaiselvi, P. Neuroprotective potential of epigallo catechin-3-gallate in PC-12 cells. Neurochem Res 38, 486–493 (2013). https://doi.org/10.1007/s11064-012-0940-9

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  • DOI: https://doi.org/10.1007/s11064-012-0940-9

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