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The Synergic Effect of Regular Exercise and Resveratrol on Kainate-Induced Oxidative Stress and Seizure Activity in Mice

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Abstract

The synergic effect of regular exercise and resveratrol, a polyphenolic compound with potent antioxidant activity, was investigated against kainate-induced seizures and oxidative stress in mice. After 6 weeks of swimming training, the total body weight decreased and the blood concentration of lactate stabilized statistically in comparison with the sedentary mice, indicate that the training program increased the aerobic resistance of mice. Kainate (30 mg/kg) evoked seizure activity 5 min after injection, and seizure activity was measured seizure rating scores every 5 min up to 2 h. As previously well known experiments, regular exercise and resveratrol (40 mg/kg, daily supplementation for 6 weeks) have an inhibitory effect on kainate-induced seizure activity and oxidative stress. In particularly, a synergistic cooperation of regular exercise and resveratrol was observed in seizure activity, mortality and oxidative stress especially in SOD activity. These results suggest that regular exercise along with an anti-convulsant agent such as resveratrol could be a more efficient method for the prevention of seizure development than exercise alone.

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References

  1. Arida RM, de Jesus Vieira A, Cavalheiro EA (1998) Effect of physical exercise on kindling development. Epilepsy Res 30:127–132

    Article  PubMed  CAS  Google Scholar 

  2. Turski WA, Cavalheiro EA, Schwarz M, Czuczwar SJ, Kleinrok Z, Turski L (1983) Limbic seizures produced by pilocarpine in rats: behavioural, electroencephalographic and neuropathological study. Behav Brain Res 9:315–335

    Article  PubMed  CAS  Google Scholar 

  3. Cavalheiro EA, Leite JP, Bortolotto ZA, Turski WA, Ikonomidou C, Turski L (1991) Long-term effects of pilocarpine in rats: structural damage of the brain triggers kindling and spontaneous recurrent seizures. Epilepsia 32:778–782

    Article  PubMed  CAS  Google Scholar 

  4. Arida RM, Scorza CA, Scorza FA, Gomes da Silva S, da Graça Naffah-Mazzacoratti M, Cavalheiro EA (2007) Effects of different types of physical exercise on the staining of parvalbumin-positive neurons in the hippocampal formation of rats with epilepsy. Prog Neuropsychopharmacol Biol Psychiatry 31:814–822

    Article  PubMed  CAS  Google Scholar 

  5. Rambo LM, Ribeiro LR, Oliveira MS, Furian AF, Lima FD, Souza MA, Silva LF, Retamoso LT, Corte CL, Puntel GO, de Avila DS, Soares FA, Fighera MR, Mello CF, Royes LF (2009) Additive anticonvulsant effects of creatine supplementation and physical exercise against pentylenetetrazol-induced seizures. Neurochem Int 55:333–340

    Article  PubMed  CAS  Google Scholar 

  6. Gobatto CA, de Mello MA, Sibuya CY, de Azevedo JR, dos Santos LA, Kokubun E (2001) Maximal lactate steady state in rats submitted to swimming exercise. Comp Biochem Physiol A Mol Integr Physiol 130:21–27

    Article  PubMed  CAS  Google Scholar 

  7. Voltarelli FA, Gobatto CA, de Mello MA (2002) Determination of anaerobic threshold in rats using the lactate minimum test. Braz J Med Biol Res 35:1389–1394

    Article  PubMed  CAS  Google Scholar 

  8. Huang SS, Tsai MC, Chih CL, Hung LM, Tsai SK (2001) Resveratrol reduction of infarct size in Long-Evans rats subjected to focal cerebral ischemia. Life Sci 69:1057–1065

    Article  PubMed  CAS  Google Scholar 

  9. Inoue H, Jiang XF, Katayama T, Osada S, Umesono K, Namura S (2003) Brain protection by resveratrol and fenofibrate against stroke requires peroxisome proliferator-activated receptor alpha in mice. Neurosci Lett 352:203–206

    Article  PubMed  CAS  Google Scholar 

  10. Sinha K, Chaudhary G, Gupta YK (2002) Protective effect of resveratrol against oxidative stress in middle cerebral artery occlusion model of stroke in rats. Life Sci 71:655–665

    Article  PubMed  CAS  Google Scholar 

  11. Gupta YK, Briyal S, Chaudhary G (2002) Protective effect of trans-resveratrol against kainic acid-induced seizures and oxidative stress in rats. Pharmacol Biochem Behav 71:245–249

    Article  PubMed  CAS  Google Scholar 

  12. Wang Q, Yu S, Simonyi A, Rottinghaus G, Sun GY, Sun AY (2004) Resveratrol protects against neurotoxicity induced by kainic acid. Neurochem Res 29:2105–2112

    Article  PubMed  CAS  Google Scholar 

  13. Gao ZB, Chen XQ, Hu GY (2006) Inhibition of excitatory synaptic transmission by trans-resveratrol in rat hippocampus. Brain Res 1111:41–47

    Article  PubMed  CAS  Google Scholar 

  14. Wu Z, Xu Q, Zhang L, Kong D, Ma R, Wang L (2009) Protective effect of resveratrol against kainate-induced temporal lobe epilepsy in rats. Neurochem Res 34:1393–1400

    Article  PubMed  CAS  Google Scholar 

  15. Das S, Das DK (2007) Anti-inflammatory responses of resveratrol. Inflamm Allergy Drug Targets 6:168–173

    Article  PubMed  CAS  Google Scholar 

  16. Sun W, Wang W, Kim J, Keng P, Yang S, Zhang H, Liu C, Okunieff P, Zhang L (2008) Anti-cancer effect of resveratrol is associated with induction of apoptosis via a mitochondrial pathway alignment. Adv Exp Med Biol 614:179–186

    Article  PubMed  CAS  Google Scholar 

  17. Shin EJ, Jeong JH, Chung YH, Kim WK, Ko KH, Bach JH, Hong JS, Yoneda Y, Kim HC (2011) Role of oxidative stress in epileptic seizures. Neurochem Int 59:122–137

    Article  PubMed  CAS  Google Scholar 

  18. Radak Z, Kumagai S, Taylor AW, Naito H, Goto S (2007) Effects of exercise on brain function: role of free radicals. Appl Physiol Nutr Metab 32:942–946

    Article  PubMed  CAS  Google Scholar 

  19. Marquezi ML, Roschel HA, dos Santa Costa A, Sawada LA, Lancha AH Jr (2003) Effect of aspartate and asparagine supplementation on fatigue determinants in intense exercise. Int J Sport Nutr Exerc Metab 3:65–75

    Google Scholar 

  20. Kim HJ, Chung JI, Lee SH, Jung YS, Moon CH, Baik EJ (2008) Involvement of endogenous prostaglandin F2alpha on kainic acid-induced seizure activity through FP receptor: the mechanism of proconvulsant effects of COX-2 inhibitors. Brain Res 1193:153–161

    Article  PubMed  CAS  Google Scholar 

  21. Souza MA, Oliveira MS, Furian AF, Rambo LM, Ribeiro LR, Lima FD, Dalla Corte LC, Silva LF, Retamoso LT, Dalla Corte CL, Puntel GO, de Avila DS, Soares FA, Fighera MR, de Mello CF, Royes LF (2009) Swimming training prevents pentylenetetrazol-induced inhibition of Na+, K+-ATPase activity, seizures, and oxidative stress. Epilepsia 50:811–823

    Article  PubMed  CAS  Google Scholar 

  22. Bleakman D, Lodge D (1998) Neuropharmacology of AMPA and kainate receptors. Neuropharmacology 37:1187–1204

    Article  PubMed  CAS  Google Scholar 

  23. Wang Q, Xu J, Rottinghaus GE, Simonyi A, Lubahn D, Sun GY, Sun AY (2002) Resveratrol protects against global cerebral ischemic injury in gerbils. Brain Res 958:439–447

    Article  PubMed  CAS  Google Scholar 

  24. Meldrum BS (2000) Glutamate as a neurotransmitter in the brain: review of physiology and pathology. J Nutr 130:1007S–1015S

    PubMed  CAS  Google Scholar 

  25. Arida RM, Scorza FA, Scorza CA, Cavalheiro EA (2009) Is physical activity beneficial for recovery in temporal lobe epilepsy? Evidences from animal studies. Neurosci Biobehav Rev 33:422–431

    Article  PubMed  Google Scholar 

  26. Arida RM, Scorza FA, dos Santos NF, Peres CA, Cavalheiro EA (1999) Effect of physical exercise on seizure occurrence in a model of temporal lobe epilepsy in rats. Epilepsy Res 37:45–52

    Article  PubMed  CAS  Google Scholar 

  27. Setkowicz Z, Mazur A (2006) Physical training decreases susceptibility to subsequent pilocarpine-induced seizures in the rat. Epilepsy Res 71:142–148

    Article  PubMed  Google Scholar 

  28. Ramsden M, Berchtold NC, Patrick Kesslak J, Cotman CW, Pike CJ (2003) Exercise increases the vulnerability of rat hippocampal neurons to kainate lesion. Brain Res 971:239–244

    Article  PubMed  CAS  Google Scholar 

  29. Bastianetto S, Zheng WH, Quirion R (2000) Neuroprotective abilities of resveratrol and other red wine constituents against nitric oxide-related toxicity in cultured hippocampal neurons. Br J Pharmacol 131:711–720

    Article  PubMed  CAS  Google Scholar 

  30. Jang JH, Surh YJ (2003) Protective effect of resveratrol on beta-amyloid-induced oxidative PC12 cell death. Free Radic Biol Med 34:1100–1110

    Article  PubMed  CAS  Google Scholar 

  31. Han YS, Zheng WH, Bastianetto S, Chabot JG, Quirion R (2004) Neuroprotective effects of resveratrol against beta-amyloid-induced neurotoxicity in rat hippocampal neurons: involvement of protein kinase C. Br J Pharmacol 141:997–1005

    Article  PubMed  CAS  Google Scholar 

  32. Virgili M, Contestabile A (2000) Partial neuroprotection of in vivo excitotoxic brain damage by chronic administration of the red wine antioxidant agent, trans-resveratrol in rats. Neurosci Lett 281:123–126

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (NRF-2012-332-2012A029).

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Authors and Affiliations

  1. Health and Exercise Science Laboratory, Institute of Sports Science, Seoul National University, Seoul, Korea

    Hee-jae Kim & Wook Song

  2. Division of Sports Industry & Science, Mokwon University, Daejeon, Korea

    Il-Kon Kim & Sok Park

  3. Department of Anatomy and Cell Biology, Collage of Medicine, Hanyang University, Seoul, Korea

    Jin Lee

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  1. Hee-jae Kim
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  2. Il-Kon Kim
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Correspondence to Sok Park.

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Kim, Hj., Kim, IK., Song, W. et al. The Synergic Effect of Regular Exercise and Resveratrol on Kainate-Induced Oxidative Stress and Seizure Activity in Mice. Neurochem Res 38, 117–122 (2013). https://doi.org/10.1007/s11064-012-0897-8

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  • DOI: https://doi.org/10.1007/s11064-012-0897-8

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